Inhibitory effects of adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf) on chemical mediator release and cytokine production in rat basophilic leukemia cells

Journal of Ethnopharmacology
Hong-Jhang ChenWenchang Chiang

Abstract

Adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) has long been used in China to treat rheumatism. We investigated the anti-allergic effects of adlay bran on rat basophilic leukemia (RBL)-2H3 cells. To evaluate the anti-allergic effects of adlay bran, the release of histamines and cytokines were measured using ELISA. To explore the mechanism of these effects, the protein expression levels were determined using western blotting. A 40.8μg/mL concentration of the ethyl acetate fraction of the ethanolic extracts of adlay bran (ABE-EtOAc) effectively inhibited mast cell degranulation. The 40-100% EtOAc/Hex subfractions of ABE-EtOAc inhibited histamine release with an IC(50) of 71-87μg/mL. Moreover, the ABE-EtOAc subfractions suppressed the secretion of interleukin (IL)-4, IL-6 and tumor necrosis factor-α in the RBL-2H3 cells, indicating that adlay bran can inhibit cytokine secretion in the late phase of the allergic reaction. In addition, adlay bran reduced the intracellular production of reactive oxygen species, inhibited the phosphorylation of Akt and decreased the expression of protein kinase C. Furthermore, six phenolic acids and one flavone were isolated. Of these compounds, luteolin showed the most potent inhibitor...Continue Reading

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Citations

Jul 20, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hong-Jhang ChenWenchang Chiang
May 21, 2013·Journal of Ethnopharmacology·Yi-hsuan LinSien-hung Yang
Feb 17, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Hsing-Yu ChenYu-Chun Chen
Feb 25, 2018·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Paula Mendonça LeiteRachel O Castilho
Nov 28, 2013·Integrative Cancer Therapies·Seong Woo YoonBharat B Aggarwal
Oct 18, 2019·Nutrients·Hsien-Tsung YaoWenchang Chiang
Jul 5, 2018·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Peirong ZhangJun Liang

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