Inhibitory Effects of Alendronate on Adhesion and Viability of Preosteoblast Cells on Titanium Discs.

European Journal of Dentistry
Charukrit LilakhunakonSomying Patntirapong

Abstract

 This study aimed to investigate the effects of alendronate (ALN; a bisphosphonate) on adhesion and viability of preosteoblasts using different cell passages on sandblasted and acid-etched (SLA) Ti surfaces.  Preosteoblast, MC3T3, cells (passage 42; P42 and passage 62; P62) were cultured with ALN (1 and 5 µM) on cell culture plate for 7 days. Cells were lifted, counted, and seeded on SLA Ti surfaces. Cells were incubated on the discs for 6 hours to examine cell adhesion by using confocal microscopy and for 24 hours to determine cell viability by using MTT assay.  ALN interfered with cell adhesion on Ti surfaces by reducing the cell number in both cell passages. Nuclei of untreated cells showed oval shape, whereas some nuclei of ALN-treated cells demonstrated crescent and condensed appearance. ALN at 1 and 5 µM significantly decreased nuclear area and perimeter in P42, while ALN at 5 µM reduced nuclear area and perimeter in P62. After 24 hours, cells (P42) grown on Ti surfaces showed decreased cell viability when culturing with 5 µM ALN.  ALN reduced cell adhesion and viability of preosteoblasts on Ti surfaces. ALN treatment seemed to exert higher inhibitory effects on nuclear shape and size as well as cell viability in lower ce...Continue Reading

References

Jan 1, 1997·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M KassemS I Rattan
Mar 30, 2004·Biomaterials·Gun-Il ImArun S Shanbhag
Feb 9, 2008·Calcified Tissue International·Aymen I IdrisStuart H Ralston
Apr 23, 2009·The Journal of International Medical Research·Y XiongL D Wu
Aug 5, 2009·Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer·Mark A ScheperTimothy F Meiller
May 4, 2010·Journal of Prosthodontic Research·Sreenivas KokaAaron Norell
Jul 3, 2010·The Journal of Oral Implantology·Talal M ZahidRobert E Cohen
May 9, 2012·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Somying PatntirapongYodhathai Satravaha
Feb 26, 2013·Oral and Maxillofacial Surgery·Cornelius von WilmowskyKarl Andreas Schlegel
Mar 26, 2014·Journal of Clinical Pharmacy and Therapeutics·S PatntirapongS Arphavasin
Sep 23, 2014·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Salvatore L RuggieroFelice O'Ryan
Sep 23, 2014·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Daniel HolzingerArno Wutzl
Nov 20, 2014·Clinical Oral Implants Research·Javier Ata-AliPablo Galindo-Moreno
Jul 9, 2017·Clinical Oral Investigations·Fernanda Gonçalves BassoCarlos Alberto de Souza Costa
Dec 28, 2017·European Journal of Dentistry·La-Ongthong VajrabhayaRudee Surarit
Nov 16, 2018·Journal of Oral & Maxillofacial Research·Rokas GelaziusGintaras Juodzbalys
Oct 22, 2019·ACS Applied Materials & Interfaces·Parisa ForoozandehMorteza Mahmoudi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Somying PatntirapongYauwaluk Suthamporn
Journal of Clinical Pharmacy and Therapeutics
S PatntirapongS Arphavasin
Physical Review. B, Condensed Matter
W Y Ching, B N Harmon
Acta Odontologica Scandinavica
Tormod B KrügerJanne E Reseland
© 2021 Meta ULC. All rights reserved