Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication.

The Journal of Biological Chemistry
Norikazu IchihashiK Sekimizu

Abstract

DnaA protein activity, the initiator of chromosomal DNA replication in bacteria, is regulated by acidic phospholipids such as phosphatidylglycerol (PG) or cardiolipin (CL) via facilitation of the exchange reaction of bound adenine nucleotide. Total lipid isolated from exponentially growing Staphylococcus aureus cells facilitated the release of ATP bound to S. aureus DnaA protein, whereas that from stationary phase cells was inert. Fractionation of total lipid from stationary phase cells revealed that the basic phospholipid, lysylphosphatidylglycerol (LPG), inhibited PG- or CL-facilitated release of ATP from DnaA protein. There was an increase in LPG concentration during the stationary phase. A fraction of the total lipid from stationary phase cells of an integrational deletion mprF mutant, in which LPG was lost, facilitated the release of ATP from DnaA protein. A zwitterionic phospholipid, phosphatidylethanolamine, also inhibited PG-facilitated ATP release. These results indicate that interaction of DnaA protein with acidic phospholipids might be regulated by changes in the phospholipid composition of the cell membrane at different growth stages. In addition, the mprF mutant exhibited an increased amount of origin per cell in v...Continue Reading

References

Feb 25, 1975·International Journal of Cancer. Journal International Du Cancer·G SteeleO Lannerstad
Jul 15, 1992·The Biochemical Journal·T MizushimaK Sekimizu
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Y Yung, A Kornberg
Jan 30, 1987·Journal of Chromatography·A A EntezamiK E Daugherty
Apr 1, 1968·The Biochemical Journal·N ShawW L Koostra
Feb 14, 1968·Journal of Molecular Biology·S Cooper, C E Helmstetter
Dec 2, 1965·Biochimica Et Biophysica Acta·U M Houtsmuller, L L van Deenen
Jul 1, 1968·Biochimica Et Biophysica Acta·J KawanamiH Otsuka
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·W Xia, W Dowhan
Mar 1, 1994·Biochimica Et Biophysica Acta·K Skarstad, E Boye
Jan 1, 1995·Methods in Enzymology·K SkarstadE Boye
Feb 16, 1996·The Journal of Biological Chemistry·T MizushimaK Sekimizu
Apr 1, 1997·Journal of Bacteriology·J MajkaJ Zakrzewska-Czerwińska
Jul 1, 1997·Biological & Pharmaceutical Bulletin·H KatayamaK Sekimizu
Jan 15, 1998·Progress in Nucleic Acid Research and Molecular Biology·N Sueoka
Jun 6, 1998·Journal of Bacteriology·W Fischer, D Arneth-Seifert
Mar 27, 2001·EMBO Reports·E BoyeK Skarstad
Apr 26, 2001·Microbiology·J A Lindsay, S J Foster
Feb 1, 2002·Journal of Biochemistry·Kohji YamamotoMurty Madiraju

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Mar 14, 2012·The Journal of Biological Chemistry·Yosuke OmaeChikara Kaito
Feb 26, 2008·Journal of Biochemistry·Kohji YamamotoMurty V V S Madiraju
May 26, 2006·Antimicrobial Agents and Chemotherapy·Lisa FriedmanJared A Silverman
Dec 30, 2006·Journal of Bacteriology·Chikara Kaito, Kazuhisa Sekimizu
Dec 14, 2004·Microbiology and Molecular Biology Reviews : MMBR·Evelyn ZientzRoy Gross
May 5, 2011·Microbial Pathogenesis·Chikara KaitoKazuhisa Sekimizu
Mar 11, 2016·Scientific Reports·Yasuhiko MatsumotoKazuhisa Sekimizu
Mar 15, 2016·Drug Discoveries & Therapeutics·Chikara Kaito
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Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Nobuyuki KawamuraKazuhisa Sekimizu
Nov 8, 2006·Molecular Microbiology·Chikara KaitoKazuhisa Sekimizu
Jan 21, 2012·Wiley Interdisciplinary Reviews. RNA·Kiley Dare, Michael Ibba
Apr 22, 2015·The FEBS Journal·Tatsuhiko KyumaChikara Kaito
Nov 3, 2004·Biochimica Et Biophysica Acta·William DowhanMikhail Bogdanov
Jan 18, 2015·International Journal of Medical Microbiology : IJMM·Sebastian KuhnAndreas Peschel
Nov 19, 2011·FEMS Microbiology Letters·Shinya MiyazakiChikara Kaito
Feb 3, 2006·FEMS Microbiology Letters·Nagamoto MuraiKazuhisa Sekimizu
May 14, 2016·Molecular Microbiology·Assaf KatzMichael Ibba
May 2, 2017·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kazuhisa Sekimizu
Jan 1, 2012·Life·Vic Norris, Patrick Amar
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yumi HashimotoAkiko Shiratsuchi

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