Inhibitory Effects of Berberine Hydrochloride on Trichophyton mentagrophytes and the Underlying Mechanisms

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Chen Wen XiaoGuo Lian Bao

Abstract

T. mentagrophytes can infect all mammals, including rabbits, causing serious infections with remarkable economic losses for rabbit farmers. Berberine is an alkaloid that is effective against a variety of microbial infections such as T. mentagrophytes. Growth curve by dry weight determination and in-vivo antifungal assay were carried out to clarify the inhibitory effect of berberine hydrochloride against T. mentagrophytes. Transcriptomics analyses were also carried out for better understanding of the underlying mechanisms. The growth rate of T. mentagrophytes was significantly higher in control condition than under berberine hydrochloride or clotrimazole for 60 h. The growth rate of T. mentagrophytes was significantly slighter higher in berberine condition (1 mg) than under clotrimazole for 46 h. T. mentagrophytes seriously shrunk after berberine or clotrimazole treatment, as observed by TEM and in SEM. Significant recovery was evident in three berberine groups on day 6 compared with the DMSO group. Results from transcriptomics analyses showed 18,881 identified unigenes, including 18,754 and 12,127 in the NT and SwissProt databases. Among these, 12,011, 9174, and 11,679 unigenes belonged to 3 Gene Ontology (GO), 43 KEGG, and 25 ...Continue Reading

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Citations

Oct 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dongdong WangAtanas G Atanasov

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Datasets Mentioned

BETA
GSE80604
SRP073785

Methods Mentioned

BETA
Illumina Sequencing
transmission electron microscopy
scanning electron microscopy
RNA-seq
PCR
chip

Software Mentioned

GraphPad Prism
Illumina Sequencing
GraphPad
RSEM
Trinity
MISA
BLAST
Bowtie2
DEseq

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