Inhibitory Effects of Chrysanthemum boreale Essential Oil on Biofilm Formation and Virulence Factor Expression of Streptococcus mutans

Evidence-based Complementary and Alternative Medicine : ECAM
Beom-Su KimYong-Ouk You

Abstract

The aim of the study was to evaluate the antibacterial activity of essential oil extracted from Chrysanthemum boreale (C. boreale) on Streptococcus mutans (S. mutans). To investigate anticariogenic properties, and bacterial growth, acid production, biofilm formation, bacterial adherence of S. mutans were evaluated. Then gene expression of several virulence factors was also evaluated. C. boreale essential oil exhibited significant inhibition of bacterial growth, adherence capacity, and acid production of S. mutans at concentrations 0.1-0.5 mg/mL and 0.25-0.5 mg/mL, respectively. The safranin staining and scanning electron microscopy results showed that the biofilm formation was also inhibited. The result of live/dead staining showed the bactericidal effect. Furthermore, real-time PCR analysis showed that the gene expression of some virulence factors such as gtfB, gtfC, gtfD, gbpB, spaP, brpA, relA, and vicR of S. mutans was significantly decreased in a dose dependent manner. In GC and GC-MS analysis, seventy-two compounds were identified in the oil, representing 85.42% of the total oil. The major components were camphor (20.89%), β-caryophyllene (5.71%), α-thujone (5.46%), piperitone (5.27%), epi-sesquiphellandrene (5.16%), α-pi...Continue Reading

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Citations

Jul 15, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Eduardo José CrevelinAntônio Eduardo Miller Crotti
Sep 21, 2016·International Journal of Molecular Sciences·Shanshan LiuHuancai Lin

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Methods Mentioned

BETA
scanning electron microscopy
Polymerase Chain Reaction
PCR
GTFase

Software Mentioned

statistical package for social sciences ( SPSS

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