Inhibitory effects of glucosamine on endotoxin-induced uveitis in Lewis rats

Investigative Ophthalmology & Visual Science
Yun-Hsiang ChangJiann-Torng Chen

Abstract

Glucosamine sulfate (GS) is a naturally occurring sugar that exerts immunosuppressive effects in vitro and in vivo. The authors investigated whether GS modulates the inflammatory reaction in endotoxin-induced uveitis (EIU) of rats and the mechanisms by which it exerts its effects. Two-hundred micrograms of lipopolysaccharide (LPS) was injected subcutaneously into Lewis rats to induce EIU. Doses of GS (10, 100, or 1000 mg/kg) were divided into three aliquots and administered intraperitoneally 30 minutes before LPS injection, concurrently with LPS injection, and 30 minutes after LPS injection. Twenty-four hours after LPS injection, aqueous humor was collected for cell counting and measurement of protein concentration. Immunohistochemical staining of the iris-ciliary body was performed to evaluate the effects of GS on intercellular adhesion molecule (ICAM)-1 and nuclear factor (NF)-kappaB activation and to demonstrate macrophage infiltration. The effects of various doses of GS pretreatment were also examined using a mouse macrophage cell line (RAW264.7 cells) and LPS stimulation. Levels of prostaglandin (PG)-E2 and nitric oxide (NO) were determined. Expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 were measure...Continue Reading

Citations

Oct 12, 2001·Immunology Letters·M CarringtonS J O'Brien
Sep 4, 2012·Clinical Ophthalmology·Ama Sadaka, Gian Paolo Giuliari
Jul 18, 2014·BioMed Research International·Yi-Hao ChenJiann-Torng Chen
Jan 5, 2011·Respiratory Physiology & Neurobiology·Ricardo FernándezPaula P Cortes
Aug 1, 2013·American Journal of Respiratory Cell and Molecular Biology·Kun-Han ChuangYuh-Lin Wu
Aug 23, 2020·Indian Journal of Ophthalmology·Mehmet BalbabaYesari Eröksüz

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