PMID: 8461426Feb 1, 1993Paper

Inhibitory effects of horse serum on immunoassay of horse ferritin

The Journal of Veterinary Medical Science
K OrinoK Watanabe

Abstract

The effects of horse serum on the immunoassay of horse ferritin were investigated using two sandwich enzyme-linked immunosorbent assay (ELISA) systems. In System A, affinity-purified antibody to horse spleen ferritin and its conjugate with alkaline phosphatase were used as the first and second antibodies, respectively. In System B, whole antiserum and its conjugate with the enzyme were used. The recoveries of horse spleen ferritin added to horse sera were very low in either system (50-71% in System A; 42-79% in System B). However, heat treatment of the sera at 75 degrees C for 15 min improved the recoveries (90-96%) in System A, whereas the recoveries in System B were not sufficiently improved by the same treatment (75-83%). The apparent concentrations of ferritin in adult and newborn horse sera increased after heat treatment of the samples. From these results, it is concluded that horse serum contains the heat-unstable substance(s) which inhibits the immunoassay of horse ferritin.

Citations

Jan 1, 2011·Journal of Equine Science·Takushi OhyaKoichi Orino
Mar 28, 2009·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Hirofumi SakamotoKoichi Orino
Jul 7, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Aya UsamiKoichi Orino
Sep 4, 2007·The Veterinary Journal·Koichi Orino, Kiyotaka Watanabe
Feb 19, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K OrinoK Watanabe
Sep 9, 2006·Journal of Dairy Science·K OrinoK Watanabe
Jan 1, 2011·Journal of Equine Science·Masafumi HashimotoKoichi Orino
Feb 21, 2002·The Journal of Veterinary Medical Science·Masami InagakiKiyotaka Watanabe
Jul 18, 2003·The Journal of Veterinary Medical Science·Motohiro KikuchiKiyotaka Watanabe
Nov 15, 2012·The Journal of Veterinary Medical Science·Seishiro ChikazawaSei-ichi Higuchi
Dec 30, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Nariaki SaitoKoichi Orino
Sep 1, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·K KakutaK Watanabe
Jun 16, 2011·Poultry Science·M WatanabeK Orino

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