Inhibitory effects of immunosuppressive drugs on insulin secretion from HIT-T15 cells and Wistar rat islets

B W PatyR P Robertson


Until recently, islet allotransplantation for type 1 diabetic patients has been largely unsuccessful. Previous pharmacologic studies of single drugs have suggested that one factor contributing to this poor success is toxicity of immunosuppressive drugs on transplanted islets. However, no comprehensive study of agents currently used for islet transplantation has been previously reported. Consequently, we exposed HIT-T15 cells and Wistar rat islets to various concentrations of five immunosuppressive agents for 48 and 24 hr, respectively, and measured glucose-stimulated insulin secretion during subsequent static incubations. Results are expressed as percent reduction of insulin secretion at the lower and upper limits, respectively, of plasma drug concentrations used in clinical transplantation compared with control (no drug exposure). Insulin secretion from HIT-T15 cells was significantly inhibited by 74% and 90% after exposure to methylprednisolone (P<0.05), 11% and 24% after exposure to cyclosporine (P<0.01), 60% and 83% after exposure to mycophenolate (P<0.05), 56% and 63% after exposure to sirolimus (P<0.001), and 10% and 20% after exposure to tacrolimus (P<0.001). Insulin secretion from Wistar rat islets was reduced by 0% and...Continue Reading


Jan 1, 1992·Metabolism: Clinical and Experimental·S StrasserD H Mintz
Sep 1, 1991·Transplantation·C RicordiT E Starzl
Jan 1, 1990·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A von KiparskiU Binswanger
Apr 1, 1989·Diabetes·S L GillisonD L Curry
Jan 1, 1986·Transplantation·H J HahnR Warzock
Jan 1, 1985·American Journal of Nephrology·E A FriedmanK M Butt
Dec 1, 1982·The Journal of Endocrinology·B Billaudel, B C Sutter
Feb 1, 1996·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·S FurthB Fivush
Apr 1, 1997·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·R M JindalM L Milgrom
Apr 9, 1998·Transplantation Proceedings·A M ShapiroN Kneteman
Nov 5, 1998·Diabetes Care·J B RedmonR P Robertson
Jun 17, 2000·Diabetes Care·R P RobertsonD E Sutherland

❮ Previous
Next ❯


Jul 18, 2003·Current Diabetes Reports·Dixon B Kaufman, William L Lowe
Jul 18, 2008·Current Diabetes Reports·Elizabeth Diakoff
Jul 18, 2008·Current Diabetes Reports·Cristiane B LeitãoRodolfo Alejandro
Apr 14, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ayman HyderJürgen Schrezenmeir
Mar 26, 2004·Transplantation Proceedings·T BerneyP Morel
Feb 20, 2008·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·D D LeeA S Chong
Feb 13, 2004·The New England Journal of Medicine·R Paul Robertson
Aug 11, 2010·Tissue Engineering. Part a·Michael Dean ChamberlainMichael V Sefton
Aug 24, 2006·Transplantation·Jennifer L LarsenFrederick G Hamel
Mar 14, 2007·Transplantation·Sophie Marcelli-TourvieilleMarie-Christine Vantyghem
Feb 8, 2011·Transplantation·Nadja NiclaussGeraldine Parnaud
May 23, 2012·Biochemistry Research International·Yong WangJosé Oberholzer
Apr 29, 2006·American Journal of Nephrology·Luiz Carlos Carvalho NavegantesEmmanuel A Burdmann
Jan 26, 2010·The Journal of Clinical Endocrinology and Metabolism·Michael R RickelsAli Naji
Dec 27, 2011·Critical Reviews in Biomedical Engineering·N V Krishnamurthy, Barjor Gimi
Apr 2, 2005·Upsala Journal of Medical Sciences·Göran Mattsson
Jan 30, 2014·Experimental and Clinical Transplantation : Official Journal of the Middle East Society for Organ Transplantation·Irfan A SheraM Saleem Najar
Dec 24, 2005·Expert Opinion on Biological Therapy·Shinichi MatsumotoCamillo Ricordi
Oct 27, 2007·Expert Opinion on Biological Therapy·Simona MarzoratiCamillo Ricordi
Aug 20, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M F NijhoffE J P de Koning
Nov 22, 2011·Transplantation Proceedings·T M SuszynskiK K Papas
Nov 30, 2006·Diabetes & Metabolism·A Penfornis, S Kury-Paulin
Aug 11, 2009·Transplantation Proceedings·B VodenikJ M Campistol
Nov 7, 2007·Endocrinology and Metabolism Clinics of North America·Savitha Subramanian, Dace L Trence
Sep 19, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jeremy J Heit
Aug 30, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·I Hernández-FisacJ Tamarit-Rodriguez
Nov 2, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·P G DeanM D Stegall
Nov 7, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·W TruongA M J Shapiro
Mar 30, 2007·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R GaoT Otonkoski
Jul 11, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·T BerneyJ Philippe
Aug 21, 2008·Transplant International : Official Journal of the European Society for Organ Transplantation·Thierry Berney, Antonio Secchi
Oct 9, 2012·Clinical and Experimental Immunology·V J AuerM J Stangl

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.