Inhibitory effects of interferons on pancreatic stellate cell activation

World Journal of Gastroenterology : WJG
Jan-Tido BaumertRobert Jaster

Abstract

To analyze and to compare the effects of interferon (IFN)-alpha, IFN-beta, and IFN-gamma on pancreatic stellate cell (PSC) activation in vitro and to elucidate the molecular basis of IFN action. PSCs were isolated from rat's pancreatic tissue, cultured and stimulated with recombinant rat IFNs. Cell proliferation and collagen synthesis were assessed by measuring the incorporation of 5-bromo-2'-deoxyuridine (BrdU) into DNA and (3H)-proline into acetic acid-soluble proteins, respectively. Apoptotic cells were determined by FACS analysis (sub-G1 peak method). Exhibition of the myofibroblastic PSC phenotype was monitored by immunoblot analysis of alpha-smooth muscle actin (alpha- SMA) expression. To assess the activation of signal transducer and activator of transcription (STAT), Western blots using phospho-STAT-specific antibodies were performed. In studies on STAT1 function, expression of the protein was inhibited by siRNA. IFN-beta and IFN-gamma but not IFN-alpha significantly diminished PSC proliferation and collagen synthesis. IFN-gamma was the only IFN that clearly inhibited alpha-SMA expression. Under the experimental conditions used, no enhanced rate of apoptotic cell death was observed in response to any IFN treatment. IFN-...Continue Reading

References

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Citations

Sep 3, 2009·World Journal of Gastroenterology : WJG·Anika JonitzRobert Jaster
Jun 1, 2010·Scandinavian Journal of Gastroenterology·Daniel AnsariRoland Andersson
Nov 13, 2007·Journal of Gastroenterology and Hepatology·Rupjyoti Talukdar, Rakesh K Tandon
May 30, 2017·Digestive Diseases and Sciences·Soumya Jagannath, Pramod Kumar Garg
Feb 18, 2017·World Journal of Gastroenterology : WJG·Ratnakar R BynigeriRupjyoti Talukdar
Mar 10, 2009·Journal of Gastroenterology·Atsushi Masamune, Tooru Shimosegawa
Sep 25, 2007·Biochemical Pharmacology·Robin BülowRobert Jaster
May 2, 2009·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Brit FitznerRobert Jaster

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