Inhibitory effects of macrocarpals on the biological activity of Porphyromonas gingivalis and other periodontopathic bacteria

Oral Microbiology and Immunology
H NagataS Shizukuishi

Abstract

Macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. This study examined effects of macrocarpals A, B, and C on periodontopathic bacteria, especially Porphyromonas gingivalis. Macrocarpals A, B, and C were purified from a 60% ethanol-extract of Eucalyptus globules leaves. To investigate antibacterial activity, representative periodontopathic bacteria were cultured in media with or without various amounts of macrocarpals; subsequently, the optical density at 660 nm was measured. Macrocarpal inhibition of P. gingivalis Arg- and Lys-specific proteinases was assessed by spectrofluorophotometric assay and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. The effect of macrocarpals on P. gingivalis binding to saliva-coated hydroxyapatite beads was examined with (3)H-labeled P. gingivalis. Growth of P. gingivalis was inhibited more strongly than growth of Prevotella intermedia or Prevotella nigrescens and Treponema denticola by macrocarpals, however, Actinobacillus actinomycetemcomitans and Fusobacterium nucleatum were much more resistant. Macrocarpals inhibited P. gingivalis Arg- and Lys-specific prote...Continue Reading

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Citations

Aug 5, 2008·Journal of Periodontology·Hideki NagataSatoshi Shizukuishi
Jul 3, 2010·Journal of Periodontology·Muneo TanakaSatoshi Shizukuishi
Oct 9, 2015·Journal of Periodontology·Shusuke IzuiHideki Nagata
Sep 8, 2010·The American Journal of Chinese Medicine·Kit-Man LauPing-Chung Leung
May 23, 2015·Natural Product Reports·María Jesús Durán-PeñaRosario Hernández-Galán
Jun 3, 2010·Molecular BioSystems·Sheryl B Rubin-PitelHuimin Zhao
Feb 18, 2017·Journal of Breath Research·Francisco Wilker Mustafa Gomes MunizCassiano Kuchenbecker Rösing
Aug 21, 2012·The Journal of Clinical Pediatric Dentistry·Y RamakrishnaA K Munshi
Nov 18, 2020·European Journal of Oral Sciences·Dorota DoblerThomas Schmidts

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