Inhibitory effects of magnolol and honokiol on human calcitonin aggregation

Scientific Reports
Caiao GuoKun Huang

Abstract

Amyloid formation is associated with multiple amyloidosis diseases. Human calcitonin (hCT) is a typical amyloidogenic peptide, its aggregation is associated with medullary carcinoma of the thyroid (MTC), and also limits its clinical application. Magnolia officinalis is a traditional Chinese herbal medicine; its two major polyphenol components, magnolol (Mag) and honokiol (Hon), have displayed multiple functions. Polyphenols like flavonoids and their derivatives have been extensively studied as amyloid inhibitors. However, the anti-amyloidogenic property of a biphenyl backbone containing polyphenols such as Mag and Hon has not been reported. In this study, these two compounds were tested for their effects on hCT aggregation. We found that Mag and Hon both inhibited the amyloid formation of hCT, whereas Mag showed a stronger inhibitory effect; moreover, they both dose-dependently disassembled preformed hCT aggregates. Further immuno-dot blot and dynamic light scattering studies suggested Mag and Hon suppressed the aggregation of hCT both at the oligomerization and the fibrillation stages, while MTT-based and dye-leakage assays demonstrated that Mag and Hon effectively reduced cytotoxicity caused by hCT aggregates. Furthermore, is...Continue Reading

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Citations

Oct 13, 2015·Food & Function·Kaige JiKun Huang
Jan 12, 2019·British Journal of Pharmacology·Yang LiKun Huang
Dec 28, 2019·Cancers·Chon Phin OngWei Hsum Yap
May 28, 2020·Chemistry : a European Journal·Richard LantzDeguo Du
Jun 27, 2019·BioMed Research International·Jianhong ZhangLonghuo Wu
Nov 22, 2019·Frontiers in Cell and Developmental Biology·Guru KrishnaKumar ViswanathanDaniel Segal
Mar 12, 2017·The Journal of Biological Chemistry·Federica RigoldiAlfonso Gautieri
Aug 23, 2020·Nitric Oxide : Biology and Chemistry·Huixian YeZhonghong Gao
Nov 7, 2019·The Journal of Physical Chemistry. B·Yi-Ting ChenLing-Hsien Tu

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Methods Mentioned

BETA
dynamic light scattering
fluorescence assay
isothermal titration calorimetry
dot blot
NMR
fluorescence

Software Mentioned

MicroCal ORIGIN

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