Inhibitory effects of methimazole and propylthiouracil on iodotyrosine deiodinase 1 in thyrocytes

Endocrine Journal
Aya YoshiharaKoichi Suzuki

Abstract

Methimazole (MMI) and propylthiouracil (PTU) are commonly used for the treatment of Graves' disease. They share similar inhibitory effects on thyroid hormone biosynthesis by interfering with thyroid peroxidase (TPO)-mediated oxidation and organification of iodine. However, their potential effects on other thyroid functional molecules have not been explored in depth. To identify novel effects of MMI and PTU, DNA microarray analysis, real-time PCR, Western blotting, immunofluorescence staining and confocal laser scanning microscopy were performed using FRTL-5 rat thyroid cells. DNA microarray analysis indicated that both MMI and PTU suppress iodotyrosine deiodinase 1 (Iyd, Dehal1) mRNA levels. Further studies revealed that Dehal1 mRNA levels was stimulated by TSH, insulin and serum, while it was suppressed by iodine and a follicular concentration of thyroglobulin. MMI and PTU significantly suppressed Dehal1 expression induced by TSH, insulin and serum. On the other hand, although MMI suppressed Dehal1 expression in the absence of TSH, PTU only weakly suppressed Dehal1 without TSH. These results suggest that PTU and MMI may use different mechanisms to regulate Dehal1 expression, and TSH may play essential and differential roles in...Continue Reading

References

Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·K SuzukiL D Kohn
Oct 13, 2000·American Journal of Physiology. Cell Physiology·M Marinò, R T McCluskey
Feb 22, 2001·European Journal of Endocrinology·P H EngL E Braverman
Feb 1, 1958·The Journal of Clinical Endocrinology and Metabolism·L J DEGROOTJ B STANBURY
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sédami GnidehouCorinne Dupuy
May 2, 2006·The Journal of Endocrinology·Koichi Suzuki, Leonard D Kohn
Feb 27, 2007·European Journal of Endocrinology·Kerstin KrauseDagmar Fuhrer
Nov 28, 1941·Science·J B MackenzieE V McCollum
Apr 25, 2008·The New England Journal of Medicine·José C MorenoTheo J Visser
Sep 4, 2008·The Journal of Clinical Endocrinology and Metabolism·Gijs AfinkCarrie Ris-Stalpers
May 14, 2009·The Journal of Biological Chemistry·Seth R ThomasSteven E Rokita
Feb 20, 2010·Biochimie·Steven E RokitaJames A Watson
Mar 8, 2011·The Journal of Endocrinology·Koichi SuzukiHiroaki J Kimura
Apr 27, 2012·The Journal of Clinical Endocrinology and Metabolism·Agnès BurniatMarc Abramowicz
Aug 3, 2012·Thyroid : Official Journal of the American Thyroid Association·Mariko SueKoichi Suzuki
Aug 10, 2012·Thyroid : Official Journal of the American Thyroid Association·Aya YoshiharaKoichi Suzuki
Nov 21, 2013·Thyroid : Official Journal of the American Thyroid Association·Donald F Sellitti, Koichi Suzuki
Dec 22, 2016·Biochemical and Biophysical Research Communications·Kenzaburo OdaKoichi Suzuki

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Citations

Apr 14, 2021·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Gökçen BiliciGüven Erbil

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