PMID: 9178189May 1, 1997Paper

Inhibitory effects of N-acetylcysteine on superoxide anion generation in human polymorphonuclear leukocytes

The Journal of Pharmacy and Pharmacology
V VillagrasaE J Morcillo

Abstract

It has been suggested that reactive oxygen species released by activated polymorphonuclear leukocytes (PMN) in man is one mechanism of tissue injury. Therapeutic action aimed at increasing antioxidant defence mechanisms is still a clinical challenge. This study examines the activity of N-acetylcysteine, a known antioxidant, in the protection of PMN exposed in-vitro to the chemoattractant peptide fMet-Leu-Phe (FMLP), the protein kinase C activator phorbol myristate acetate or the lipid peroxidation promoter t-butyl hydroperoxide. FMLP (3-300 nM) and phorbol myristate acetate (160 pm-160 nM) induced concentration-related superoxide anion generation. Pre-treatment with N-acetylcysteine (33-333 microM) resulted in concentration-related inhibition of superoxide production induced by FMLP (30 nM) or phorbol myristate acetate (16 nM);-log IC50 values were 3.97 +/- 0.07 and 3.91 +/- 0.10, respectively. Changes in intracellular calcium ion concentration ([Ca2+]i) induced by FMLP (30 nM) were studied in fura-2-loaded human PMN. FMLP produced a transient calcium response, i.e. a peak followed by decay to a residual value above baseline. N-Acetylcysteine (333 microM) did not affect either basal [Ca2+]i values or changes in [Ca2+]i values a...Continue Reading

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Citations

Aug 7, 2002·European Journal of Pharmacology·Yukio IshiiKiyohisa Sekizawa
Apr 27, 2000·Journal of Hepatology·G BouchardB Tuchweber
Nov 30, 2004·Clinical and Experimental Pharmacology & Physiology·Shu-Jing WuChun-Ching Lin
Nov 11, 2008·Respiratory Physiology & Neurobiology·A Gomez-NiñoC Gonzalez
Apr 27, 2013·Biochimica Et Biophysica Acta·Yuval SamuniMichael Berk
May 6, 2004·Basic & Clinical Pharmacology & Toxicology·Irena ManovTheodore C Iancu
Jun 9, 2004·Ultrasound in Medicine & Biology·Hidemi HondaHiroshi Kitagawa
Apr 3, 2002·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Irena ManovTheodore C Iancu
Apr 26, 2007·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M Martinez-LosaE J Morcillo
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·Y CadroyH Plaisancié
Jun 17, 2020·Archives of Iranian Medicine·Shirinsadat BadriShahram Taheri
Dec 4, 2020·Medicina·Timur SalievDainius Pavalkis
Oct 21, 1999·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·E J MorcilloJ Cortijo

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