Inhibitory effects of polysorbate 80 on MRSA biofilm formed on different substrates including dermal tissue

Scientific Reports
Yutaka UedaShiro Jimi

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) forms biofilms on necrotic tissues and medical devices, and causes persistent infections. Surfactants act on biofilms, but their mode of action is still unknown. If used in the clinic, cytotoxicity in tissues should be minimized. In this study, we investigated the inhibitory effect of four different surfactants on MRSA biofilm formation, and found that a nonionic surfactant, polysorbate 80 (PS80), was the most suitable. The biofilm inhibitory effects resulted from the inhibition of bacterial adhesion to substrates rather than biofilm disruption, and the effective dose was less cytotoxic for 3T3 fibroblasts. However, the effects were substrate-dependent: positive for plastic, silicon, and dermal tissues, but negative for stainless-steel. These results indicate that PS80 is effective for prevention of biofilms formed by MRSA on tissues and foreign bodies. Therefore, PS80 could be used in medical practice as a washing solution for wounds and/or pretreatment of indwelling catheters.

References

Oct 1, 1987·Antimicrobial Agents and Chemotherapy·B L ProsserR Cleeland
Jun 1, 1987·Antimicrobial Agents and Chemotherapy·R C Evans, C J Holmes
Oct 1, 1993·Regulatory Toxicology and Pharmacology : RTP·J H McGeeR D Sofia
Apr 16, 2002·Molecular Microbiology·Friedrich Götz
Aug 28, 2002·International Journal of Medical Microbiology : IJMM·Philip S Stewart
Jul 6, 2004·The Journal of Biological Chemistry·Fiona M RocheTimothy J Foster
Jun 29, 2006·Molecular Microbiology·Maria ScarselliBeatrice Aricò
Oct 3, 2006·Advances in Microbial Physiology·Simon R Clarke, Simon J Foster
Oct 27, 2006·American Journal of Rhinology·Zohra BendouahMartin Desrosiers
Feb 11, 2009·The Journal of Infectious Diseases·C BlombergB Henriques-Normark
Jun 17, 2009·Cell Host & Microbe·Kimberly A KlineBirgitta Henriques-Normark
Sep 5, 2009·The Journal of Clinical Investigation·Frank R DeLeo, Henry F Chambers
May 11, 2011·Advances in Experimental Medicine and Biology·Christine Heilmann
May 20, 2011·Colloids and Surfaces. B, Biointerfaces·Hans-Curt Flemming
Dec 24, 2011·Annals of the New York Academy of Sciences·Megan R Kiedrowski, Alexander R Horswill
Mar 15, 2013·The Journal of Antimicrobial Chemotherapy·Laetitia G GarciaFrançoise Van Bambeke
Mar 20, 2013·International Wound Journal·Taro MakinoHiroyuki Ohjimi
Nov 20, 2014·Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine·Suganthan VeerachamyPrasad Kdv Yarlagadda
Sep 27, 2016·Biofouling·Rudolph E SloupChristopher M Waters

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