PMID: 11933141Apr 5, 2002Paper

Inhibitory effects of quinones on RNase H activity associated with HIV-1 reverse transcriptase

Phytotherapy Research : PTR
B S MinM Hattori

Abstract

In an effort to develop new drugs preventing the growth of human immunodeficiency virus (HIV), we developed an in vitro assay method of ribonuclease H (RNase H) activity associated with reverse transcriptase (RT) from HIV-1. Some naphthoquinones, such as 1,4-naphthoquinone (1), vitamin K(3) (2), juglone (3) and plumbagin (6), moderately inhibited RNase H activity, and others, including naphthazarin (5) and shikonins (8-9, 18-23), showed weak inhibition. Diterpenoid quinones, tanshinones (24-28), had also moderate inhibition against RNase H activity. Of these quinones, compound 1 showed the most potent inhibition on RNase H activity with a 50% inhibitory concentration (IC(50)) of 9.5 microM, together with moderate inhibition against RNA-dependent and DNA-dependent DNA polymerase (RDDP and DDDP) activities with IC(50) values of 69 and 36 microM, respectively. Compounds 3 and 5 showed significant inhibition against RDDP (IC(50) = 8 and 10 microM, respectively) and DDDP (IC(50) = 5 and 7 microM, respectively) activities. The structure-activity relationship of the naphthoquinones suggested that non-hydroxylated naphthoquinones (1 and 2) showed significant inhibition of RNase H activity, whereas 5-hydroxylated naphthoquinones (3 and ...Continue Reading

References

Aug 11, 1991·Nucleic Acids Research·P HafkemeyerU Hübscher
Jul 1, 1985·Journal of Natural Products·N KakiuchiT Okuda
Dec 1, 1989·Journal of Virology·K MoellingH Diringer
Nov 1, 1995·Journal of Natural Products·H K LeeC O Lee
Oct 31, 2000·Phytotherapy Research : PTR·G HusseinK Shimotohno

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Citations

Mar 3, 2011·Journal of Natural Medicines·Sudeep SabdeInder Pal Singh
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Galina N NikolenkoVinay K Pathak
Mar 19, 2011·Biological & Pharmaceutical Bulletin·Hong GaoChun-Yan Gao
Jan 23, 2016·Frontiers in Microbiology·Kesava Rao V KurapatiMadhavan P N Nair
Sep 29, 2009·Antiviral Research·Catherine S Adamson, Eric O Freed
Jan 7, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jemal DemmaBjörn Hellman
Apr 1, 2010·Viruses·Greg L Beilhartz, Matthias Götte
Feb 18, 2005·Natural Product Research·Yun-Sen LiJi-Jun Chen
Oct 12, 2007·Phytochemical Analysis : PCA·Tomás GrevenstukAnabela Romano
Dec 14, 2002·The Journal of Biological Chemistry·Cathryn A Shaw-ReidDaria J Hazuda
Oct 18, 2020·European Journal of Pharmacology·Revathy NadhanPriya Srinivas
Dec 29, 2021·The American Journal of Chinese Medicine·Zhenzhen MuLong Geng

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