Inhibitory mechanisms of activated matrix metalloproteinase-9 on platelet activation

European Journal of Pharmacology
Ye-Ming LeeJoen-Rong Sheu

Abstract

The intracellular mechanisms underlying the signaling pathways of activated matrix metalloproteinase-9 (MMP-9) in platelets are not yet completely understood. Therefore, the aim of this study was to further examine the effects of activated MMP-9 in preventing platelet aggregation. In this study, activated MMP-9 time-dependently (3-60 min) inhibited platelet aggregation in washed human platelet suspensions stimulated by agonists. However, activated MMP-9 had no significant effect on the binding of FITC-triflavin to the platelet glycoprotein IIb/IIIa complex. Triflavin is a specific antagonist of the glycoprotein IIb/IIIa complex purified from snake venom. Moreover, activated MMP-9 (21 and 90 ng/ml) markedly decreased the fluorescence intensity of platelet membranes tagged with diphenylhexatriene. The thrombin-evoked increase in pHi was inhibited in the presence of activated MMP-9 (21 and 90 ng/ml). In addition, activated MMP-9 (21 and 90 ng/ml) markedly reduced the electron spin resonance (ESR) signal intensity of hydroxyl radicals in collagen (1 mug/ml)-activated platelets. These results indicate that the antiplatelet activity of activated MMP-9 may involve the following pathways: (1) activated MMP-9 may initially induce confor...Continue Reading

References

Nov 1, 1995·Circulation Research·C M DolleryA M Henney
Apr 15, 1994·European Journal of Biochemistry·L IulianoF Violi
Oct 1, 1995·Current Opinion in Cell Biology·H Birkedal-Hansen
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·G R WangM E Mendelsohn
Sep 11, 1998·Journal of Medicinal Chemistry·M BaumgarthR Gericke
Jul 16, 2002·Biochimica Et Biophysica Acta·Donatella PietraforteMaurizio Minetti

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Citations

Oct 6, 2009·Critical Care : the Official Journal of the Critical Care Forum·Leonardo LorenteJosé A Páramo
Apr 15, 2014·PloS One·Leonardo LorenteJosé A Páramo
Aug 8, 2007·Thrombosis Research·María José Santos-MartínezMarek W Radomski
Apr 27, 2013·Journal of Thrombosis and Haemostasis : JTH·M RahmanH Thorlacius
May 23, 2015·Medical Hypotheses·Hassan NiknejadMona Kakavand
Apr 8, 2016·Expert Opinion on Drug Metabolism & Toxicology·Thanasekaran JayakumarJoen-Rong Sheu
Jun 1, 2012·Platelets·Aneta Wrzyszcz, Mieczyslaw Wozniak
Dec 20, 2017·Molecular Medicine Reports·Xinxing LuHaifeng Mei
Oct 9, 2021·Seminars in Thrombosis and Hemostasis·Martin J Page, Etheresia Pretorius

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