Inhibitory selectivity of canecystatin: a recombinant cysteine peptidase inhibitor from sugarcane

Biochemical and Biophysical Research Communications
Maria Luiza V OlivaFlavio Henrique-Silva

Abstract

The cDNA of a cystein peptidase inhibitor was isolated from sugarcane and expressed in Escherichia coli. The protein, named canecystatin, has previously been shown to exert antifungal activity on the filamentous fungus Trichoderma reesei. Herein, the inhibitory specificity of canecystatin was further characterized. It inhibits the cysteine peptidases from plant source papain (Ki =3.3nM) and baupain (Ki=2.1x10(-8)M), but no inhibitory effect was observed on ficin or bromelain. Canecystatin also inhibits lysosomal cysteine peptidases such as human cathepsin B (Ki=125nM), cathepsin K (Ki=0.76nM), cathepsin L (Ki=0.6nM), and cathepsin V (Ki=1.0nM), but not the aspartyl peptidase cathepsin D. The activity of serine peptidases such as trypsin, chymotrypsin, pancreatic, and neutrophil elastases, and human plasma kallikrein is not affected by the inhibitor, nor is the activity of the metallopeptidases angiotensin converting enzyme and neutral endopeptidase. This is the first report of inhibitory activity of a sugarcane cystatin on cysteine peptidases.

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Citations

Jun 15, 2012·Biomolecular NMR Assignments·Ítalo Augusto CaviniClaudia Elisabeth Munte
May 24, 2011·Molecular and Cellular Probes·Baohua HeLi He
Jan 21, 2011·Plant Physiology and Biochemistry : PPB·Phuwadol Bangrak, Wilaiwan Chotigeat
Feb 6, 2009·Biotechnology Progress·Carolina W RibeiroFlavio Henrique-Silva
Dec 7, 2005·Protein Expression and Purification·Andréia GianottiFlávio Henrique-Silva
Jul 3, 2013·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ashraf Gholizadeh
Aug 29, 2014·Environmental Science and Pollution Research International·Mateusz KędziorGrzegorz Terlecki
Feb 22, 2017·Genetics and Molecular Biology·Thais P SouzaMarcio C Silva-Filho
Jun 13, 2017·Journal of Dental Research·A C SantiagoF Henrique-Silva
May 21, 2019·Journal of Biomolecular Structure & Dynamics·Fakhra AminBilqees Bano
Dec 8, 2020·International Journal of Biological Macromolecules·Priscila Yumi Tanaka ShibaoFlavio Henrique-Silva

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