Initial cell plating density affects properties of human primary synovial mesenchymal stem cells

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Kaori NakamuraIchiro Sekiya

Abstract

Synovial mesenchymal stem cells (MSCs) appear to be an attractive cell source in cartilage and meniscus regeneration because of their high proliferative and chondrogenic potentials. Two methods are used to culture synovial nucleated cells in the preparation of primary synovial MSCs. In one method, the cells are plated at low density to make cell colonies. In the other method, the cells are plated at high density. We investigated the effects of initial cell density on proliferation, surface markers, and multipotentiality, including chondrogenesis in primary synovial MSCs. Human synovium was obtained from the knee joints of patients with osteoarthritis after total knee arthroplasty. Immediately after enzyme digestion, the synovial nucleated cells were plated in densities of 103 , 104 , or 105  cells/60-cm2 dish and cultured for 14 days. Proliferation, surface markers, chondrogenesis, adipogenesis, and calcification were examined in three populations. The cell colonies were distinct in the 103  cells/dish group, faint in the 104  cells/dish group, and obscure in the 105  cells/dish group. The total number of cells/dish was positively related to plating density, whereas the fold increase was negatively related to plating density (n...Continue Reading

References

May 1, 1976·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu F GorskaiaN N Kulagins
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ichiro SekiyaDarwin J Prockop
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ichiro SekiyaDarwin J Prockop
Jul 4, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Karin A CorsiJohnny Huard
Dec 31, 2014·Mediators of Inflammation·Claudia CasniciOrnella Marelli
Feb 17, 2015·Osteoarthritis and Cartilage·Y NakagawaI Sekiya
May 1, 2015·Clinical Orthopaedics and Related Research·Ichiro SekiyaHideyuki Koga
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory

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Citations

May 3, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Farshid GuilakJohnny Huard
Nov 12, 2020·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Hajime UtsunomiyaSoshi Uchida
Jun 15, 2021·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Ichiro SekiyaNobutake Ozeki

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