Initial characterization of sheep T-cell growth factor and its species-restricted activity on human, rat, and mouse cells

Cellular Immunology
L S EnglishM Whitehurst

Abstract

Sheep T-cell growth factor (TCGF) was prepared from concanavalin A-activated sheep peripheral blood cells and subsequently characterized by ammonium sulfate precipitation, gel exclusion chromatography, and isoelectric focussing. The TCGF was found in the 60-80% ammonium sulfate fraction and was shown to have an apparent molecular weight of 32,500 and an isoelectric point in the range pI 5.2-5.5. The ability of the sheep TCGF to promote proliferation of activated human, sheep, mouse, and rat cells was compared with that of human TCGF prepared by phytohemagglutinin stimulation of lymphocytes from multiple donors and TCGF prepared from concanavalin A-stimulated rat and mouse spleen cells. Human TCGF was found to act across all species barriers, rat TCGF supported the growth of cells of all species except human, and mouse only promoted the growth of activated mouse and rat cells. Sheep TCGF was unique in being unable to support the growth of any cells except autologous cells.

References

Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L M Neckers, J Cossman
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W Mier, R C Gallo
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G Di Sabato

Citations

Nov 23, 1987·Journal of Immunological Methods·H MartensenM W Bentzon
Sep 1, 1987·Veterinary Immunology and Immunopathology·K A Knisley, L D Pearson
Dec 1, 1989·Veterinary Immunology and Immunopathology·B ArunachalamG C Ram
Feb 9, 1999·Veterinary Immunology and Immunopathology·D ThiagarajanM P Bansal
Oct 11, 1990·Nucleic Acids Research·J C GoodallL Hall

Related Concepts

Metazoa
Cell Division Phases
Molecular Sieve Chromatography
RU 49637
Isoelectric Focusing
Leukocytes
Dall Sheep
T-Lymphocyte
Mouse, Swiss
Rats, Laboratory

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