Initial heme uptake from albumin by short-term cultured rat hepatocytes is mediated by a transport mechanism differing from that of other organic anions

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
C M NoyerA W Wolkoff

Abstract

Although it is known that circulating heme accumulates in liver cells, the process by which heme enters hepatocytes is only partly understood. Hemopexin and a putative hemopexin receptor on hepatocyte membranes may mediate the uptake process. However, whether there are sufficient hemopexin receptors on rat hepatocytes to account for the bulk of heme entering cells is unknown. It is likely that heme may be transferred directly from albumin with the help of a plasma membrane heme transporter. To clarify the transport mechanism of heme into liver cells, we studied the uptake by short-term cultured rat hepatocytes of 55Fe-heme incubated with rat serum albumin. In these cells, the initial uptake of 55Fe-heme at 37 degrees C was five- to eightfold higher than that at 4 degrees C, linear for at least 5 minutes, and saturable. The Km of heme uptake was 0.95 +/- 0.27 micromol/L, and the Vmax was 0.12 +/- 0.01 pmol/min/mg protein (n = 3). Neither isosmotic substitution of sucrose for NaCl in the medium nor adenosine triphosphate (ATP) depletion, perturbations that are known to reduce uptake of bilirubin, sulfobromophthalein (BSP), and taurocholate, had any influence on 55Fe-heme uptake. In addition, heme uptake was not reduced in the pre...Continue Reading

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Citations

Jun 4, 2002·DNA and Cell Biology·Emanuela Tolosano, Fiorella Altruda
Aug 10, 2000·Archives of Biochemistry and Biophysics·Y ShanH L Bonkovsky
Dec 13, 2006·FEBS Letters·Gladys O Latunde-DadaAndrew T McKie
Feb 21, 2006·Trends in Biochemical Sciences·Gladys O Latunde-DadaAndrew T McKie
Jan 18, 2011·Biochimica Et Biophysica Acta·Anwar A Khan, John G Quigley
Sep 7, 2005·Cell·Majid ShayeghiAndrew T McKie
Jun 14, 2014·Frontiers in Pharmacology·Tamara Korolnek, Iqbal Hamza
Aug 31, 2000·The Journal of Biological Chemistry·C M Maya-MonteiroP L Oliveira
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·M T WorthingtonC L Berg
Jun 20, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gabriel NúñezMiguel P Soares

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