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Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique

The Biochemical Journal

Sep 1, 1975

Michael Alan Titheradge, H G Coore

PMID: 2157

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Abstract

An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates dep...read more

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Mitochondria, Liver
Metazoa
Hydrogen-Ion Concentration
Coumaric Acids
Pyruvates
Nitriles
Cinnamates
Rats, Laboratory
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  • Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique

    The Biochemical Journal

    Sep 1, 1975

    Michael Alan Titheradge, H G Coore

    PMID: 2157

    DOI:

    Abstract

    An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates dep...read more

    Mentioned in this Paper

    Mitochondria, Liver
    Metazoa
    Hydrogen-Ion Concentration
    Coumaric Acids
    Pyruvates

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