Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Scott CameronWilliam N Hunter

Abstract

The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P6(1/5)22 and unit-cell parameters a = 86.44, c = 221.56 A, gamma = 120 degrees. The Matthews coefficient, VM, of 2.7 A3 Da(-1) corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Dec 10, 1999·Natural Product Reports·H Seto, T Kuzuyama
Sep 5, 2002·Chembiochem : a European Journal of Chemical Biology·Anna WoschekFriedrich Hammerschmidt
Dec 21, 2002·Molecular Microbiology·Ahmed El ZoeibyRoger C Levesque
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·A MirakhurM J Walshaw

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Citations

Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Karen McLuskeyWilliam N Hunter
Mar 4, 2006·Journal of Inorganic Biochemistry·Ian J CliftonChristopher J Schofield

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