PMID: 702647Oct 1, 1978Paper

Initiation and maintenance of persistent infection by respiratory syncytial virus

Journal of Virology
C R P'ringleP Malloy

Abstract

Propagation of cells infected with temperature-sensitive (ts) mutants of respiratory syncytial (RS) virus at nonpermissive temperature (39 degrees C) resulted in cytolytic, abortive, or persistent infection, depending on the mutant used to initiate infection. Five mutants from complementation group B produced cytolytic or abortive infections, whereas a single mutant (ts1) from group D and a noncomplbmenting mutant produced persistent infections. The persistently infected culture initiated by mutant ts1 (RS ts1/BS-C-1) has been maintained in serial culture for greater than 100 transfers, and infectious-center assays and immunofluorescent staining indicated that all cells harbored the RS virus genome. RS ts1/BS-C-1 cultures were resistant to superinfection by homologous and some heterologous viruses, and interferon-like activity against some heterologous viruses was present in the culture medium. Small amounts (0.002 to 0.2 PFU/cell) of infectious virus were present in the culture fluid, but autointerfering defective particles were not detected. This released virus formed small plaques and produced persistent infection of BS-C-1 cells at 37 degrees C. The RS ts1/BS-C-1 cells contained abundant RS virus antigen internally, but lit...Continue Reading

References

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Citations

Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Changying LiPramod K Srivastava
Jan 1, 1985·Archives of Virology·E J Stott, G Taylor
Jan 1, 1980·Microbiology and Immunology·Y KimuraT Matsumoto
Jan 1, 2013·Current Pediatrics Reports·Peter Mastrangelo, Richard G Hegele
Oct 3, 2008·The Journal of Infectious Diseases·Dora EstripeautAsuncion Mejias
Mar 1, 1996·Veterinary Immunology and Immunopathology·A K Sharma, Z Woldehiwet
Nov 1, 1980·Virology·H B Gimenez, R W Compans
Sep 1, 1994·The Journal of Infection·W H Van der PoelJ T Van Oirschot

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