PMID: 9440515Jan 24, 1998Paper

Initiation and velocity of chromosome replication in Escherichia coli B/r and K-12

Journal of Bacteriology
M BipatnathH Bremer

Abstract

The macromolecular composition and a number of parameters affecting chromosome replication were examined over a range of exponential growth rates in two common Escherichia coli strains, B/r and K-12 AB1157. Based on improved measurements of DNA after treatment of exponential cultures with rifampin, the cell mass per chromosomal replication origin (initiation mass) and the time required to replicate the chromosome from origin to terminus (C period) were determined. For these two strains, the initiation mass approached values of 8 x 10(-10) and 10 x 10(-10) units of optical density (at 460 nm) of culture mass per oriC, respectively, at growth rates above 1 doubling/h (at 37 degrees C). The amount of protein per oriC decreased with increasing growth rate for AB1157 and remained nearly constant for the B/r strain. The C period decreased for both strains in an essentially identical manner from about 70 min at 0.6 doublings/h to about 33 min at 3 doublings/h. From the initiation mass and C period, relative or absolute copy numbers for genes with known map locations can be accurately determined at different growth rates. At growth rates above 2 doublings/h, when chromosomes are highly branched, genes near the origin are about threefol...Continue Reading

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Citations

Nov 9, 2001·The Journal of Biological Chemistry·Keith E ShearwinJ Barry Egan
Jan 25, 2002·Journal of Bacteriology·Li-Fong LeeCarton W Chen
Jun 18, 2002·Molecular Microbiology·Jan OlssonKurt Nordström
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