PMID: 9188631Jul 1, 1997Paper

Initiation of DNA synthesis by human papillomavirus E7 oncoproteins is resistant to p21-mediated inhibition of cyclin E-cdk2 activity

Journal of Virology
M N Ruesch, Laimonis Laimins

Abstract

The E6 and E7 proteins from the high-risk human papillomaviruses (HPVs) bind and inactivate the tumor suppressor proteins p53 and Rb, respectively. In HPV-positive cells, expression of E6 proteins from high-risk types results in increased turnover of p53, which leads to an abrogation of p21-mediated G1/S arrest in response to DNA-damaging agents. In contrast, keratinocytes which express E7 alone have increased levels of p53 but, interestingly, also fail to undergo a G1/S arrest. We investigated the mechanism by which E7 bypasses this p21 arrest by using both keratinocytes which stably express E7 as well as U20S cells which stably or transiently express E7. We observed that E7 does not affect the induction of p21 synthesis by p53. While glutathione S-transferase (GST)-E7 bound a low level of in vitro-translated p21, we were unable to detect E7 and p21 in the same complex by GST-E7 binding assays or immunoprecipitations from cell extracts. Furthermore, E7 did not prevent p21-mediated inhibition of cyclin E kinase activity. In keratinocytes expressing E7, increased levels of p53, p21, and cyclin E, as well as increased cyclin E kinase activity, were observed. To determine if this increase in cyclin E activity was necessary for E7'...Continue Reading

References

Apr 1, 1992·Journal of Virology·W C PhelpsP M Howley
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D V HeckK Münger
Jan 1, 1990·Journal of Virology·S WatanabeK Yoshiike
May 1, 1995·Molecular and Cellular Biology·C L WuJ A Lees
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·C MisseroG P Dotto
Jan 1, 1994·Advances in Cancer Research·K H Vousden
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C SardetR A Weinberg
Nov 18, 1994·Cell·C J Sherr
Aug 1, 1994·Genes & Development·H ZhangD Beach
Nov 15, 1994·Genes & Development·D GinsbergD M Livingston
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Q QinP D Adams
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·D R LowyJ T Schiller
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Wu, A J Levine
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·G W DemersD A Galloway
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J SlebosK R Cho
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T D KessisK R Cho

❮ Previous
Next ❯

Citations

Jun 26, 2001·International Journal of Experimental Pathology·C Swanton, N Jones
Aug 8, 2006·American Journal of Clinical Pathology·Synne KnoppRuth Holm
Dec 24, 2010·World Journal of Surgical Oncology·Michael StamatakosKonstantinos Kontzoglou
Jan 30, 2004·The Journal of Biological Chemistry·Kyoko Tsukiyama-KoharaMichinori Kohara
Apr 21, 2007·Uirusu·Tadahito Kanda, Iwao Kukimoto
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·K Smith-McCuneJ M Bishop
Aug 23, 2003·Oncogene·Wei PanSrilata Bagchi
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S SongP F Lambert
Aug 29, 2006·World Journal of Gastroenterology : WJG·Nicola FiedlerStephan Schaefer
Jun 26, 2001·International Journal of Experimental Pathology·H R McMurrayD J McAnce
Aug 12, 1999·Annals of Medicine·S M Syrjänen, K J Syrjänen
May 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R Donnellan, R Chetty
Sep 21, 2002·Molecular and Cellular Biology·Thomas F WestbrookDennis J McCance
Jun 19, 2008·Cancer Research·Valia T MihaylovaGary M Kupfer
Jun 5, 2013·Virology·Ann Roman, Karl Munger
May 29, 2002·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·S Wadler
Nov 15, 2008·Virology·Margaret E McLaughlin-Drubin, Karl Münger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.