Initiation of mRNA translation in prokaryotes
No abstract listed.
DNA-directed in vitro synthesis of beta-galactosidase: requirement for formylation of methionyl-tRNAf
Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity
The anti-Shine-Dalgarno region in Escherichia coli 16S ribosomal RNA is not essential for the correct selection of translational starts
Cross-linking of initiation factor IF3 to Escherichia coli 30S ribosomal subunit by trans-diamminedichloroplatinum(II): characterization of two cross-linking sites in 16S rRNA; a possible way of functioning for IF3
A single base change in the Shine-Dalgarno region of 16S rRNA of Escherichia coli affects translation of many proteins
Specialized ribosome system: preferential translation of a single mRNA species by a subpopulation of mutated ribosomes in Escherichia coli
Escherichia coli initiation factor 3 protein binding to 30S ribosomal subunits alters the accessibility of nucleotides within the conserved central region of 16S rRNA
A translational enhancer derived from tobacco mosaic virus is functionally equivalent to a Shine-Dalgarno sequence
An additional ribosome-binding site on mRNA of highly expressed genes and a bifunctional site on the colicin fragment of 16S rRNA from Escherichia coli: important determinants of the efficiency of translation-initiation
Mutation at position 791 in Escherichia coli 16S ribosomal RNA affects processes involved in the initiation of protein synthesis
Alternative occupancy of a dual ribosomal binding site by mRNA affected by translation initiation factors
Site-directed mutagenesis of Escherichia coli translation initiation factor IF1. Identification of the amino acid involved in its ribosomal binding and recycling
Ribosomal affinity and translational initiation in Escherichia coli. In vitro investigations using translational initiation regions of differing efficiencies from the atp operon
Ribosomal protein S1 and initiation factor IF3 do not promote the ribosomal binding of approximately 19-nucleotide-long mDNA and mRNA models
Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT
AUU-to-AUG mutation in the initiator codon of the translation initiation factor IF3 abolishes translational autocontrol of its own gene (infC) in vivo
Molecular cloning and sequence of the Bacillus stearothermophilus translational initiation factor IF2 gene
Identification, cloning and sequence of the Streptococcus faecium infB (translational initiation factor IF2) gene
Study of the interaction of Escherichia coli methionyl-tRNA synthetase with tRNAfMet using chemical and enzymatic probes
The unusual translational initiation codon AUU limits the expression of the infC (initiation factor IF3) gene of Escherichia coli
Mutants of Escherichia coli formylmethionine tRNA: a single base change enables initiator tRNA to act as an elongator in vitro
Binding of Escherichia coli protein synthesis initiation factor IF1 to 30S ribosomal subunits measured by fluorescence polarization
Escherichia coli formylmethionine tRNA: mutations in GGGCCC sequence conserved in anticodon stem of initiator tRNAs affect initiation of protein synthesis and conformation of anticodon loop
Translational efficiency of the Escherichia coli adenylate cyclase gene: mutating the UUG initiation codon to GUG or AUG results in increased gene expression
Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors
Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane
The ribosome binding sites recognized by E. coli ribosomes have regions with signal character in both the leader and protein coding segments
Fluorescence polarization studies of the interaction of Escherichia coli protein synthesis initiation factor 3 with 30S ribosomal subunits
The topographical localization of IF3 on Escherichia coli 30 S ribosomal subunits as a clue to its way of functioning
Transformation of carrot tissues derived from proembryogenic suspension cells: A useful model system for gene expression studies in plants
Nucleotide sequence of the ilvH-fruR gene region of Escherichia coli K12 and Salmonella typhimurium LT2
Identification and characterization of PilS, an essential regulator of pilin expression in Pseudomonas aeruginosa
In-vitro translation of mitochondrial mRNAs by yeast mitochondrial ribosomes is hampered by the lack of start-codon recognition
Physiological effects of translation initiation factor IF3 and ribosomal protein L20 limitation in Escherichia coli
An inhibitor of elongation factor G (EF-G) GTPase present in the ribosome wash of Escherichia coli: a complex of initiation factors IF1 and IF3?
Cloning and sequence analysis of the cDNA for bovine mitochondrial translational initiation factor 2
Site-directed mutagenesis and NMR spectroscopic approaches to the elucidation of the structure-function relationships in translation initiation factors IF1 and IF3
Eukaryotic initiation factors eIF-2 and eIF-3: interactions, structure and localization in ribosomal initiation complexes
Ribosome-mRNA contact sites at different stages of translation initiation as revealed by cross-linking of model mRNAs
Construction and characterization of an Escherichia coli mutant with a deletion of the metZ gene encoding tRNA (f1Met)
Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella
Three tandemly repeated structural genes encoding tRNA(f1Met) in the metZ operon of Escherichia coli K-12
mRNA secondary structure can greatly affect production of recombinant phospholipase A(2) toxins in bacteria
Mutation of Thr445 and Ile500 of initiation factor 2 G-domain affects Escherichia coli growth rate at low temperature
Organization of the Thermus thermophilus nusA/infB operon and overexpression of the infB gene in Escherichia coli
Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique
Expression in E. coli and purification of Thermus thermophilus translation initiation factors IF1 and IF3
Translation during cold adaptation does not involve mRNA-rRNA base pairing through the downstream box
Initiation factor IF 2 binds to the alpha-sarcin loop and helix 89 of Escherichia coli 23S ribosomal RNA
Identification of the ribosome binding sites of translation initiation factor IF3 by multidimensional heteronuclear NMR spectroscopy
Interaction of IF2 with the ribosomal GTPase-associated center during 70S initiation complex formation
The antibiotic kasugamycin mimics mRNA nucleotides to destabilize tRNA binding and inhibit canonical translation initiation
Cloning and characterization of hIF2, a human homologue of bacterial translation initiation factor 2, and its interaction with HIV-1 matrix
Initiation factors of protein biosynthesis in bacteria and their structural relationship to elongation and termination factors
Insertion domain within mammalian mitochondrial translation initiation factor 2 serves the role of eubacterial initiation factor 1
Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site
Translation of mRNAs with degenerate initiation triplet AUU displays high initiation factor 2 dependence and is subject to initiation factor 3 repression
The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif
Selective stimulation of translation of leaderless mRNA by initiation factor 2: evolutionary implications for translation
Domains of eIF1A that mediate binding to eIF2, eIF3 and eIF5B and promote ternary complex recruitment in vivo
Ribosome-binding site interference caused by Shine-Dalgarno-like nucleotide sequences in Escherichia coli cells
Molecular cloning of a ribonuclease H (RNase HI) gene from an extreme thermophile Thermus thermophilus HB8: a thermostable RNase H can functionally replace the Escherichia coli enzyme in vivo
Lack of a 5' non-coding region in Tn1721 encoded tetR mRNA is associated with a low efficiency of translation and a short half-life in Escherichia coli
Interaction of the Escherichia coli fdhF mRNA hairpin promoting selenocysteine incorporation with the ribosome
Enhancement of translation by the epsilon element is independent of the sequence of the 460 region of 16S rRNA
A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli
Role of the N- and C-terminal extensions on the activity of mammalian mitochondrial translational initiation factor 3
Crucial contribution of the multiple copies of the initiator tRNA genes in the fidelity of tRNA(fMet) selection on the ribosomal P-site in Escherichia coli
GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis
Molecular structure of the Frankia spp. nifD-K intergenic spacer and design of Frankia genus compatible primer
PilS and PilR, a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa
Translational initiation at the coat-protein gene of phage MS2: native upstream RNA relieves inhibition by local secondary structure
Vitamin B12 repression of the cob operon in Salmonella typhimurium: translational control of the cbiA gene
Molecular characterisation of the pifC gene encoding translation initiation factor 3, which is required for normal photosynthetic complex formation in Rhodobacter sphaeroides NCIB 8253
RNase activity of polynucleotide phosphorylase is critical at low temperature in Escherichia coli and is complemented by RNase II
Complementation analysis of the cold-sensitive phenotype of the Escherichia coli csdA deletion strain
Localization of cold shock proteins to cytosolic spaces surrounding nucleoids in Bacillus subtilis depends on active transcription
Depletion of free 30S ribosomal subunits in Escherichia coli by expression of RNA containing Shine-Dalgarno-like sequences
Correlations between Shine-Dalgarno sequences and gene features such as predicted expression levels and operon structures
Isolation and characterization of ribosomes and translation initiation factors from the gram-positive soil bacterium Streptomyces lividans
Structural and spectroscopic properties of a reaction center complex from the chlorosome-lacking filamentous anoxygenic phototrophic bacterium Roseiflexus castenholzii
Physical and functional interaction between the eukaryotic orthologs of prokaryotic translation initiation factors IF1 and IF2
A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15
Sulfolobus solfataricus translation initiation factor 1 stimulates translation initiation complex formation
Computational exploration of structural hypotheses for an additional sequence in a mammalian mitochondrial protein
Direct interaction of the N-terminal domain of ribosomal protein S1 with protein S2 in Escherichia coli
A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants
Disulfide linkages in Plasmodium falciparum plasmepsin-i are essential elements for its processing activity and multi-milligram recombinant production yield
Efficient in vitro translational termination in Escherichia coli is constrained by the orientations of the release factor, stop signal and peptidyl-tRNA within the termination complex
Suppressor mutations in Escherichia coli methionyl-tRNA formyltransferase: role of a 16-amino acid insertion module in initiator tRNA recognition
Enhancement of translation by the downstream box does not involve base pairing of mRNA with the penultimate stem sequence of 16S rRNA
How Escherichia coli can bias the results of molecular cloning: preferential selection of defective genomes of hepatitis C virus during the cloning procedure
Induced fit of a peptide loop of methionyl-tRNA formyltransferase triggered by the initiator tRNA substrate
Specific, efficient, and selective inhibition of prokaryotic translation initiation by a novel peptide antibiotic
SSoNDelta and SSoNDeltalong: two thermostable esterases from the same ORF in the archaeon Sulfolobus solfataricus?
Isolation and identification of fxsA, an Escherichia coli gene that can suppress F exclusion of bacteriophage T7
Expression of penicillin G acylase from the cloned pac gene of Escherichia coli ATCC11105. Effects of pacR and temperature
Cis-acting signals controlling translational initiation in the thermophilic archaeon Sulfolobus solfataricus
In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level
Recent developments of engineered translational machineries for the incorporation of non-canonical amino acids into polypeptides
Interaction of fMet-tRNA(fMet) with the C-terminal domain of translational initiation factor IF2 from Bacillus stearothermophilus
70S-scanning initiation is a novel and frequent initiation mode of ribosomal translation in bacteria
Reinitiation of protein synthesis in Escherichia coli can be induced by mRNA cis-elements unrelated to canonical translation initiation signals
Characterization of the domains of E. coli initiation factor IF2 responsible for recognition of the ribosome
The fMet-tRNA binding domain of translational initiation factor IF2: role and environment of its two Cys residues
The C-A mismatch base pair and the single-strand terminus in the E. coli initiator tRNA(fMet) acceptor stem adopt unusual conformations
Domain of E. coli translational initiation factor IF2 homologous to lambda cI repressor and displaying DNA binding activity
On the target of a novel class of antibiotics, oxazolidinones, active against multidrug-resistant Gram-positive bacteria
Discrimination of 5'-terminal start codons by translation initiation factor 3 is mediated by ribosomal protein S1
Modulation of ribosomal recruitment to 5'-terminal start codons by translation initiation factors IF2 and IF3
Tandem translation of Bacillus subtilis initiation factor IF2 in E. coli. Over-expression of infBB.su in E. coli and purification of alpha- and beta-forms of IF2B.su
Translation initiation factor 3 regulates switching between different modes of ribosomal subunit joining
Activation of prokaryotic translation by antisense oligonucleotides binding to coding region of mRNA
Genomic and transcriptomic analysis of the streptomycin-dependent Mycobacterium tuberculosis strain 18b
Initial bridges between two ribosomal subunits are formed within 9.4 milliseconds, as studied by time-resolved cryo-EM
On how many fundamental kinds of cells are present on Earth: looking for phylogenetic traits that would allow the identification of the primary lines of descent
In vivo studies on putative Shine-Dalgarno sequences of the halophilic archaeon Halobacterium salinarum
Functional analysis of the translation factor aIF2/5B in the thermophilic archaeon Sulfolobus solfataricus
Translation initiation with GUC codon in the archaeon Halobacterium salinarum: implications for translation of leaderless mRNA and strict correlation between translation initiation and presence of mRNA
Control of translation initiation involves a factor-induced rearrangement of helix 44 of 16S ribosomal RNA
Requirements for translation re-initiation in Escherichia coli: roles of initiator tRNA and initiation factors IF2 and IF3
Mutational analysis in patients with neuromuscular disorders: Detection of mitochondrial deletion and double mutations in the MT-ATP6 gene
Escherichia coli cold-shock gene profiles in response to over-expression/deletion of CsdA, RNase R and PNPase and relevance to low-temperature RNA metabolism
Tandem translation of E. coli initiation factor IF2 beta: purification and characterization in vitro of two active forms
MG428 is a novel positive regulator of recombination that triggers mgpB and mgpC gene variation in Mycoplasma genitalium
Nucleotides of tRNA governing the specificity of Escherichia coli methionyl-tRNA(fMet) formyltransferase
Gradient-based optimization of kernel-target alignment for sequence kernels applied to bacterial gene start detection
A bacterial homolog YciH of eukaryotic translation initiation factor eIF1 regulates stress-related gene expression and is unlikely to be involved in translation initiation fidelity
Both forms of translational initiation factor IF2 (alpha and beta) are required for maximal growth of Escherichia coli. Evidence for two translational initiation codons for IF2 beta
Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector-Coding Sequence Junction
Characterization of the elastase inhibitor properties of alpha-crystallin and the water-insoluble fraction from bovine lens
Mis-translation of a computationally designed protein yields an exceptionally stable homodimer: implications for protein engineering and evolution
Enhancing the efficiency of cell-free protein synthesis through the polymerase-chain-reaction-based addition of a translation enhancer sequence and the in situ removal of the extra amino acid residues
The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization
Proteomic analysis of Vibrio metschnikovii under cold stress using a quadrupole Orbitrap mass spectrometer
Molecular cloning and high-level expression of human polymerase beta cDNA and comparison of the purified recombinant human and rat enzymes
An unexpected type of ribosomes induced by kasugamycin: a look into ancestral times of protein synthesis?
How initiation factors maximize the accuracy of tRNA selection in initiation of bacterial protein synthesis
Binding of Escherichia coli initiation factor IF2 to 30S ribosomal subunits: a functional role for the N-terminus of the factor
Ribosomal crystallography: initiation, peptide bond formation, and amino acid polymerization are hampered by antibiotics
The translational fidelity function of IF3 during transition from the 30 S initiation complex to the 70 S initiation complex
Roles of the N- and C-terminal domains of mammalian mitochondrial initiation factor 3 in protein biosynthesis
Thermodynamics of GTP and GDP binding to bacterial initiation factor 2 suggests two types of structural transitions
A single mammalian mitochondrial translation initiation factor functionally replaces two bacterial factors
Protein biogenesis in Archaea: addressing translation initiation using an in vitro protein synthesis system for Haloferax volcanii
A possible contribution of mRNA secondary structure to translation initiation efficiency in Lactococcus lactis
Initiation factors IF1 and IF2 synergistically remove peptidyl-tRNAs with short polypeptides from the P-site of translating Escherichia coli ribosomes
Post-transcriptional control of bacteriophage T4 gene 25 expression: mRNA secondary structure that enhances translational initiation
Development of a high-throughput scintillation proximity assay for the identification of C-domain translational initiation factor 2 inhibitors
A tRNA-derived fragment competes with mRNA for ribosome binding and regulates translation during stress
Translational activation in coliphage Qbeta: on a polycistronic messenger RNA, repression of one gene can activate translation of another
Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli
Fluorescence study of the topology of messenger RNA bound to the 30S ribosomal subunit of Escherichia coli
Structure-function analysis of Escherichia coli translation initiation factor IF3: tyrosine 107 and lysine 110 are required for ribosome binding
Nature of the ribosomal mRNA track: analysis of ribosome-binding sites containing different sequences and secondary structures
Initiation factor 3-induced structural changes in the 30 S ribosomal subunit and in complexes containing tRNA(f)(Met) and mRNA
Prokaryotic translation initiation factor IF3 is an elongated protein consisting of two crystallizable domains
Strand specific RNA-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in Listeria monocytogenes
Non-canonical Escherichia coli transcripts lacking a Shine-Dalgarno motif have very different translational efficiencies and do not form a coherent group
Effect of the amino acid attached to Escherichia coli initiator tRNA on its affinity for the initiation factor IF2 and on the IF2 dependence of its binding to the ribosome.
The C-terminal subdomain (IF2 C-2) contains the entire fMet-tRNA binding site of initiation factor IF2
Lysine 207 as the site of cross-linking between the 3'-end of Escherichia coli initiator tRNA and methionyl-tRNA formyltransferase.
An AUG initiation codon, not codon-anticodon complementarity, is required for the translation of unleadered mRNA in Escherichia coli
Identification of mammalian mitochondrial translational initiation factor 3 and examination of its role in initiation complex formation with natural mRNAs.
Synonymous mutations make dramatic contributions to fitness when growth is limited by a weak-link enzyme
Novel Translation Initiation Regulation Mechanism in Escherichia coli ptrB Mediated by a 5'-Terminal AUG
Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomes
Biochemical characterization of pathogenic mutations in human mitochondrial methionyl-tRNA formyltransferase.
Incorporation characteristics of exogenous 15N-labeled thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine into bacterial DNA
Mutants of Escherichia coli initiator tRNA defective in initiation. Effects of overproduction of methionyl-tRNA transformylase and the initiation factors IF2 and IF3.
Regulation of the activity of chloroplast translational initiation factor 3 by NH2- and COOH-terminal extensions.
The nucleic acid melting activity of Escherichia coli CspE is critical for transcription antitermination and cold acclimation of cells
Interplay of methionine tRNAs with translation elongation factor Tu and translation initiation factor 2 in Escherichia coli.
Mammalian mitochondrial initiation factor 2 supports yeast mitochondrial translation without formylated initiator tRNA.
Evidence for the translation initiation of leaderless mRNAs by the intact 70 S ribosome without its dissociation into subunits in eubacteria.
Interaction between eukaryotic initiation factors 1A and 5B is required for efficient ribosomal subunit joining
Fluorophores at the N terminus of nascent chloramphenicol acetyltransferase peptides affect translation and movement through the ribosome
Disassembly of the Staphylococcus aureus hibernating 100S ribosome by an evolutionarily conserved GTPase
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Archaeal translation initiation revisited: the initiation factor 2 and eukaryotic initiation factor 2B alpha-beta-delta subunit families
Investigation of the translation-initiation factor IF2 gene, infB, as a tool to study the population structure of Streptococcus agalactiae
Expression, purification and ligand binding properties of the recombinant translation initiation factor (PeIF5B) from Pisum sativum
Important role of the amino acid attached to tRNA in formylation and in initiation of protein synthesis in Escherichia coli.
The interdomain linker of Escherichia coli initiation factor IF3: a possible trigger of translation initiation specificity
Unusual dicistronic expression from closely spaced initiation codons in an umbravirus subgenomic RNA
Construction of anti-codon table of the plant kingdom and evolution of tRNA selenocysteine (tRNASec )
Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
Correlation of 16S ribosomal DNA signature sequences with temperature-dependent growth rates of mesophilic and psychrotolerant strains of the Bacillus cereus group
Metabolic Flux of N10-Formyltetrahydrofolate Plays a Critical Role in the Fidelity of Translation Initiation in Escherichia coli.
Mutations at two invariant nucleotides in the 3'-minor domain of Escherichia coli 16 S rRNA affecting translational initiation and initiation factor 3 function.
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