PMID: 2236066Nov 1, 1990Paper

Initiation of rolling-circle replication in pT181 plasmid: initiator protein enhances cruciform extrusion at the origin

Proceedings of the National Academy of Sciences of the United States of America
P NoirotR P Novick

Abstract

Plasmid pT181 DNA secondary structures have been analyzed in vitro by nuclease S1 digestion and in vivo by bromoacetaldehyde treatment. A cruciform structure occurring at the pT181 replication origin in vitro is greatly enhanced by the binding of the plasmid-encoded initiator protein RepC. In vivo a DNA secondary structure also existed in the replication origin. Its frequency of formation was correlated with efficiency of RepC utilization. These data suggest that cruciform extrusion at the origin is involved in initiation of pT181 rolling-circle replication. A neighboring DNA structure influences the conformation of the origin in vivo.

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Citations

Oct 1, 1996·Journal of Cellular Biochemistry·C E PearsonM Zannis-Hadjopoulos
Sep 24, 2002·Journal of Cellular Biochemistry·David AlvarezMaria Zannis-Hadjopoulos
Feb 1, 2012·Journal of the American Society for Mass Spectrometry·Helga Dögg FlosadóttirOddur Ingólfsson
Dec 16, 1998·Trends in Biochemical Sciences·R P Novick
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L A DempseyS A Khan
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·J LaufsB Gronenborn
Mar 31, 2010·Nucleic Acids Research·Natali FiliJustin E Molloy
Mar 16, 2007·Journal of Virology·Tobias SteinfeldtAnnette Mankertz
Aug 10, 2000·Journal of Virology·J R Brister, N Muzyczka
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·David BikardDidier Mazel
Aug 6, 2011·BMC Molecular Biology·Václav BrázdaCheryl Arrowsmith
Jul 16, 2010·The EMBO Journal·Céline LootDidier Mazel
Nov 10, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Rika Kusumoto-MatsuoIwao Kukimoto
Mar 1, 1992·Plasmid·S J Projan, R P Novick
Apr 22, 2004·Journal of Molecular Biology·Elena A OussatchevaVladimir N Potaman
Jul 7, 1998·Journal of Molecular Biology·L S ShlyakhtenkoY L Lyubchenko
Aug 5, 2016·Nucleic Acids Research·Cesar L PastranaFernando Moreno-Herrero
Dec 9, 2017·Frontiers in Microbiology·Stephen M KwongNeville Firth
Jan 21, 2018·Scientific Reports·Algirdas ToleikisJustin E Molloy
Feb 1, 1993·Molecular & General Genetics : MGG·G del SolarM Espinosa
Jan 24, 2002·The Journal of Biological Chemistry·Olivia NovacMaria Zannis-Hadjopoulos

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