PMID: 976261Sep 15, 1976

Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation

European Journal of Biochemistry
M FresnoD Vazquez

Abstract

In order to elucidate the mechanism of action of inhibitors that block the initiation of protein synthesis in mammalian systems, we have studied the following steps: (a) formation of the ternary complex Met-tRNAr-IF-E2-GTP, (b) binding of the initiator Met-tRNAf to the 40-S ribosomal subunit in the presence of initiation factors and dependent or not on the addition of mRNA, (c) formation of the initiation complex with 80-S ribosomes and (d) formation of the first peptide bond. Adrenochrome, aurintricarboxylic acid, polydextran sulphate, pyrochatechol violet and showdomycin block the formation of the ternary complex Met-tRNAf-IF-E2-GTP. Edeine A1, aurintricarboxylic acid and polydextran sulphate block the binding of the mRNA to the 40-S ribosomal subunit. Pactamycin induces the formation of stable smaller initiation complexes which are unable to go through the subsequent steps of initiation. Stimulation of the binding of the initiator Met-tRNAf to the 80-S ribosome in the presence of initiation factors is observed with sparsomycin and antibiotics of the sesquiterpene family (verrucarin A, trichodermin and trichothecin). However, these antibiotics block the reaction of the bound Met-tRNAf with puromycin. Narciclasine has no effec...Continue Reading

References

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Citations

May 1, 1980·Die Naturwissenschaften·K H Nierhaus, H G Wittmann
May 1, 1991·Journal of Neuroscience Methods·G R Thériaul, D Gauthier
May 7, 2013·The Journal of Biological Chemistry·Denis G KnyazevPeter Pohl
Jul 11, 2006·Molecular Biology of the Cell·Julia Schaletzky, Tom A Rapoport
Sep 28, 2001·The EMBO Journal·V EvdokimovaNahum Sonenberg
Sep 25, 1990·Nucleic Acids Research·C D Prescott, H U Göringer
Jun 1, 1980·Plant Physiology·A Matilla, G Nicolás
Jan 1, 1977·European Journal of Biochemistry·M FresnoD Vazquez
Feb 1, 1978·European Journal of Biochemistry·M Fresno, D Vazquez
Mar 1, 1978·European Journal of Biochemistry·C J Carter, M Cannon
Aug 1, 1979·European Journal of Biochemistry·F Sánchez-MadridJ P Ballesta
Mar 1, 1981·European Journal of Biochemistry·F Sánchez-MadridJ P Ballesta
Jan 27, 2007·BMC Cancer·André O von BuerenMichael A Grotzer
Aug 1, 2013·Microvascular Research·Omar Saeed Ali Al-SalahiNarazah Mohd Yusoff
Aug 20, 2003·Biopolymers·Anat BashanAda Yonath
Nov 26, 1990·FEBS Letters·S K SmailovO N Denisenko
Oct 1, 1984·Medicinal Research Reviews·L Carrasco, D Vazquez
May 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin QiJing Li
Dec 8, 2020·Biochemistry. Biokhimii︠a︡·S E DmitrievK A Lashkevich

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