PMID: 976261Sep 15, 1976

Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation

European Journal of Biochemistry
M FresnoD Vazquez


In order to elucidate the mechanism of action of inhibitors that block the initiation of protein synthesis in mammalian systems, we have studied the following steps: (a) formation of the ternary complex Met-tRNAr-IF-E2-GTP, (b) binding of the initiator Met-tRNAf to the 40-S ribosomal subunit in the presence of initiation factors and dependent or not on the addition of mRNA, (c) formation of the initiation complex with 80-S ribosomes and (d) formation of the first peptide bond. Adrenochrome, aurintricarboxylic acid, polydextran sulphate, pyrochatechol violet and showdomycin block the formation of the ternary complex Met-tRNAf-IF-E2-GTP. Edeine A1, aurintricarboxylic acid and polydextran sulphate block the binding of the mRNA to the 40-S ribosomal subunit. Pactamycin induces the formation of stable smaller initiation complexes which are unable to go through the subsequent steps of initiation. Stimulation of the binding of the initiator Met-tRNAf to the 80-S ribosome in the presence of initiation factors is observed with sparsomycin and antibiotics of the sesquiterpene family (verrucarin A, trichodermin and trichothecin). However, these antibiotics block the reaction of the bound Met-tRNAf with puromycin. Narciclasine has no effec...Continue Reading


Feb 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C NombelaW C Merrick
Mar 17, 1975·Biochemical and Biophysical Research Communications·C NombelaF Anderson
Jul 8, 1975·Biochemical and Biophysical Research Communications·B V Treadwell, W G Robinson
Aug 24, 1973·Biochimica Et Biophysica Acta·L Carrasco, D Vazquez
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L M Cashion, W M Stanley
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Suzuki, I H Goldberg
Jul 11, 1972·Biochemical and Biophysical Research Communications·Y C ChenK K Gupta
Jan 1, 1973·Annual Review of Biochemistry·R Haselkorn, L B Rothman-Denes
Mar 23, 1974·FEBS Letters·D Vazquez
Oct 20, 1966·Biochemical and Biophysical Research Communications·P Leder, H Bursztyn


May 1, 1980·Die Naturwissenschaften·K H Nierhaus, H G Wittmann
May 1, 1991·Journal of Neuroscience Methods·G R Thériaul, D Gauthier
May 7, 2013·The Journal of Biological Chemistry·Denis G KnyazevPeter Pohl
Jul 11, 2006·Molecular Biology of the Cell·Julia Schaletzky, Tom A Rapoport
Sep 28, 2001·The EMBO Journal·V EvdokimovaNahum Sonenberg
Sep 25, 1990·Nucleic Acids Research·C D Prescott, H U Göringer
Jun 1, 1980·Plant Physiology·A Matilla, G Nicolás
Jan 1, 1977·European Journal of Biochemistry·M FresnoD Vazquez
Feb 1, 1978·European Journal of Biochemistry·M Fresno, D Vazquez
Mar 1, 1978·European Journal of Biochemistry·C J Carter, M Cannon
Aug 1, 1979·European Journal of Biochemistry·F Sánchez-MadridJ P Ballesta
Mar 1, 1981·European Journal of Biochemistry·F Sánchez-MadridJ P Ballesta
Jan 27, 2007·BMC Cancer·André O von BuerenMichael A Grotzer
Aug 1, 2013·Microvascular Research·Omar Saeed Ali Al-SalahiNarazah Mohd Yusoff
Aug 20, 2003·Biopolymers·Anat BashanAda Yonath
Nov 26, 1990·FEBS Letters·S K SmailovO N Denisenko
Oct 1, 1984·Medicinal Research Reviews·L Carrasco, D Vazquez
May 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin QiJing Li
Dec 8, 2020·Biochemistry. Biokhimii︠a︡·S E DmitrievK A Lashkevich

Related Concepts

Guanosine Triphosphate
Peptide Chain Initiation, Translational
Peptide Initiation Factors
Transfer RNA

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.