Initiation sites for translation of sindbis virus 42S and 26S messenger RNAs.

Cell
R CanceddaM J Schlesinger

Abstract

Sindbis virus 26S RNA is the principal species of virus-specific RNA found in the infected cell; it is derived from a one third segment of virion 42S RNA. When translated in cell-free extracts from mouse ascites cells or rabbit reticulocytes, 26S RNA directed the synthesis primarily of the 33,000 dalton virus capsid protein, and the protein products were in the form of free peptides rather than peptidyl-tRNA. In contrast, the polypeptides synthesized in either extract in response to Sindbis virus 42S RNA were heterogeneous, ranging in molecular weight from 33,000 to 190,000, and were largely in the form of peptidyl-tRNA. The number of independent initiation sites on the 26S and 42S RNAs was determined by analyzing a tryptic digest of reaction products labeled with yeast N-formyl-35S-methionyl-tRNAFmet. The 26S RNA appeared to contain a single initiation site, and this site could also be found in varying amounts in different preparations of 42S RNA. However, a second initiation site, distinct from that of 26S RNA, was the major site in 42S virion RNA. These results suggest that 42S virion RNA contains two potential sites for initiation of protein synthesis. Only one of these may be active, however, and it is postulated that the ...Continue Reading

References

Sep 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·M F Jacobson, D Baltimore
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·B F Oberg, A J Shatkin
Jun 25, 1974·Journal of Molecular Biology·D T Simmons, J H Strauss
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·D SwanP Leder
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M J McDowellH F Lodish
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D S Shih, P Kaesberg
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Cancedda, M J Schlesinger
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·T G Morrison, H F Lodish
Jan 1, 1973·Annual Review of Biochemistry·R Haselkorn, L B Rothman-Denes
Mar 1, 1973·Journal of Virology·H Rosemond, T Sreevalsan
Jun 1, 1973·Journal of Virology·M J Schlesinger, S Schlesinger
Apr 15, 1973·Journal of Molecular Biology·M H SchreierP F Spahr
Oct 1, 1973·The Journal of General Virology·M J MorserD C Burke
Aug 29, 1970·Nature·D HousmanH F Lodish
Dec 1, 1965·Journal of Molecular Biology·M S BretscherJ D Smith
Nov 9, 1970·Biochemical and Biophysical Research Communications·M OsbornH F Lodish
Jan 1, 1971·The Journal of General Virology·P DobosP Faulkner
Mar 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·E WECKER, E SCHONNE

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Citations

Aug 1, 1992·Protein Science : a Publication of the Protein Society·C RyanM J Schlesinger
Jan 1, 1979·Archives of Virology·J D MartinR W Beck
Jan 1, 1977·Medical Microbiology and Immunology·R W Schlesinger
May 23, 1978·Biochimica Et Biophysica Acta·N GlanvilleL Kääriäinen
Jun 1, 1982·Virology·J R BellJ H Strauss
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B E Lachmi, L Kääriäinen
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·N GlanvilleA E Smith
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A F PurchioR L Erikson
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J Kamine, J M Buchanan
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S C InglisJ W Almond
Feb 15, 1979·European Journal of Biochemistry·R Cancedda, A J Shatkin
Oct 22, 2002·Journal of Virology·Wei ZhangMichael G Rossmann
Jul 1, 1976·Virology·M Bracha, M J Schlesinger
Dec 1, 1980·Virology·F J Fuller, P I Marcus
Jan 15, 1982·Journal of Molecular Biology·C M Rice, J H Strauss
Jan 1, 1980·Annals of the New York Academy of Sciences·H F LodishM Porter
Jan 1, 1980·Pharmacology & Therapeutics·L Carrasco, A E Smith
Feb 1, 1979·Journal of Virology·D DohnerS Schlesinger
Apr 1, 1977·Journal of Virology·B Lachmi, L Kääriäinen
May 1, 1977·Journal of Virology·H Brzeski, S I Kennedy
Jan 1, 1978·Journal of Virology·R M Buller, J A Rose

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