Initiator recognition in a primitive eukaryote: IBP39, an initiator-binding protein from Trichomonas vaginalis

Molecular and Cellular Biology
David R ListonP J Johnson

Abstract

While considerable progress has been made in understanding the mechanisms of transcription in higher eukaryotes, transcription in single-celled, primitive eukaryotes remains poorly understood. Promoters of protein-encoding genes in the parasitic protist Trichomonas vaginalis, which represents one of the deepest-branching eukaryotic lineages, have a bipartite structure with gene-specific regulatory elements and a conserved core promoter encompassing the transcription start site. Core promoters in T. vaginalis appear to consist solely of a highly conserved initiator (Inr) element that is both a structural and a functional homologue of its metazoan counterpart. Using DNA affinity chromatography, we have isolated an Inr-binding protein from T. vaginalis. Cloning of the gene encoding the Inr binding protein identified a novel 39-kDa protein (IBP39). We show that IBP39 binds to both double and single Inr motifs found in T. vaginalis genes and that binding requires the conserved nucleotides necessary for Inr function in vivo. Analyses of the cloned IBP39 gene revealed no homology at the protein sequence level with identified proteins in other organisms or the presence of known DNA-binding domains. The relationship between IBP39 and In...Continue Reading

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Citations

Apr 3, 2003·International Journal for Parasitology·Stepanka VanacovaPatricia J Johnson
Apr 23, 2003·Molecular and Biochemical Parasitology·Diana Ortiz, Patricia J Johnson
Aug 30, 2003·Molecular and Biochemical Parasitology·Audrey O T LauPatricia J Johnson
Oct 30, 2008·Nucleic Acids Research·Augusto Simoes-BarbosaPatricia J Johnson
Apr 3, 2012·Eukaryotic Cell·Vanessa FuentesImelda López-Villaseñor
Mar 26, 2003·Annual Review of Biochemistry·Stephen T Smale, James T Kadonaga
Mar 6, 2010·Journal of Biomedicine & Biotechnology·Consuelo GomezMario A Rodríguez
Jun 23, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·J C Torres-Romero, R Arroyo
Aug 2, 2006·Molecular and Biochemical Parasitology·Audrey O T LauPatricia J Johnson
May 16, 2006·International Journal for Parasitology·Jacqueline A UpcroftPeter Upcroft
Apr 23, 2011·Research in Microbiology·Alias Smith, Patricia Johnson
Jun 20, 2015·BioMed Research International·Rossana ArroyoJaime Ortega-López
Nov 19, 2003·Cell·Maria A SchumacherPatricia J Johnson
Jan 6, 2017·Cellular Microbiology·Tomas PachanoNatalia de Miguel
Oct 11, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·José Luis VillalpandoMaria Elizbeth Alvarez-Sánchez
Sep 2, 2019·Parasitology Research·Olivia Parra-MarínImelda López-Villaseñor
May 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ayelen LizarragaNatalia de Miguel
Feb 19, 2021·Molecular Microbiology·Ayelen LizarragaNatalia de Miguel
Aug 11, 2020·Molecular and Biochemical Parasitology·Olivia Parra-MarínImelda López-Villaseñor

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