Injectable and bioactive methylcellulose hydrogel carrying bone mesenchymal stem cells as a filler for critical-size defects with enhanced bone regeneration.

Colloids and Surfaces. B, Biointerfaces
Lizhi DengLi-Ming Zhang

Abstract

As a soluble cellulose derivative, methylcellulose (MC), can be used to construct thermosensitive hydrogels. However, a pure MC gel is generally considered an inert material that is inadequate for cell survival. We adopted an environmentally friendly method to fabricate a nano-hydroxyapatite (nHA) hybrid MC hydrogel. Rheology results showed that the addition of nHA increased the gelation temperature. Furthermore, the live/dead assay confirmed that the addition of nHA improved the survival of bone marrow-derived mesenchymal stem cells (BMSCs) inside the gel. In addition, ARS staining indicated that the presence of nHA stimulated osteogenic differentiation. Finally, in vivo cranial defect experiments showed improved remediation efficiency when using the nHA hybrid MC hydrogel to carry BMSCs.

References

Mar 19, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Y XuL Li
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Jiang LiuyunLiao Jianguo
Aug 14, 2012·International Journal of Biological Macromolecules·Mrinal Kanti BainDipankar Chattopadhyay
Sep 29, 2012·Nature Protocols·Patrick P SpicerAntonios G Mikos
May 18, 2016·Advanced Materials·Takayuki NonoyamaJian Ping Gong
Dec 17, 2017·International Journal of Biological Macromolecules·Min Hee KimWon Ho Park
Apr 5, 2019·International Journal of Biological Macromolecules·Cheisy D F MoreiraMarivalda M Pereira
May 18, 2019·International Journal of Biological Macromolecules·Tian GongYurong Guo

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