Injectable cartilaginous template transformed BMSCs into vascularized bone

Scientific Reports
Xiaoke FengYimin Zhao

Abstract

Regeneration of alveolar bone for dental implant remains a major issue, partifcularly for patients suffering from severe bone adsorption and irregular socket trauma. Recapitulating embryological development is becoming an attractive approach for engineer organ or three-dimensional tissues from stem cells. In this study, we aimed to develop an injectable "cartilaginous" graft with adequate mechanical resistance and ideal bone remodelling potential. The cartilaginous graft was composed of a particulate decellularised cartilage matrix (PDCM), chondrogenically primed bone mesenchymal stem cell (BMSC) bricks (CB), and enriched platelet-rich plasma (P) gel. In immunodeficient mice, we found that angiogenesis occurred quickly inside PDCM-CB-P constructs after implantation, thereby improving tissue survival and bone formation. In rabbit tibia bone defects around implants, we confirmed that CBs not only transformed into bone tissue rapidly, but also significantly promoted bone remodelling and replacement of PDCM, thus realising osseointegration of dental implants within 3 months. In conclusion, CBs exhibited the potential for endochondral ossification in vivo, and application of a cartilaginous template composed of PDCM, CB, and P provi...Continue Reading

References

Jun 23, 2012·International Journal of Oral Science·Yan-Qi YangA Bakr M Rabie
Jan 8, 2013·Calcified Tissue International·Christa Maes
Jan 10, 2013·Trends in Biotechnology·Kim E M BendersJos Malda
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Celeste ScottiIvan Martin
Nov 22, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chelsea S BahneyRalph S Marcucio
Jun 12, 2014·Journal of Tissue Engineering and Regenerative Medicine·Emmet M ThompsonFergal J O'Brien
Sep 4, 2014·Journal of Dental Research·R C ZhengJ H Lee
Sep 28, 2014·Bone·Adam PapadimitropoulosIvan Martin
Oct 16, 2014·Tissue Engineering. Part a·Debby GawlittaWouter J A Dhert
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Paul E BourgineIvan Martin
Mar 18, 2015·Birth Defects Research. Part C, Embryo Today : Reviews·Nicola C FosterAlicia J El Haj
Jun 18, 2015·Frontiers in Bioengineering and Biotechnology·Marietta HerrmannMauro Alini
Sep 25, 2015·Clinical Implant Dentistry and Related Research·Özgür ErdoganPakpoom Kheolamai
Oct 11, 2015·Journal of Biomedical Materials Research. Part a·Jakub JaroszewiczStanisław Moskalewski

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Citations

Aug 21, 2021·Frontiers in Cell and Developmental Biology·Zihao LiLunhao Bai

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Methods Mentioned

BETA
Scanning electron microscopy
pull-out
Assay
bone marrow aspiration

Software Mentioned

SPSS

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