Injectable dual-gelling cell-laden composite hydrogels for bone tissue engineering

Biomaterials
Tiffany N VoAntonios G Mikos

Abstract

The present work investigated the osteogenic potential of injectable, dual thermally and chemically gelable composite hydrogels for mesenchymal stem cell (MSC) delivery in vitro and in vivo. Composite hydrogels comprising copolymer macromers of N-isopropylacrylamide were fabricated through the incorporation of gelatin microparticles (GMPs) as enzymatically digestible porogens and sites for cellular attachment. High and low polymer content hydrogels with and without GMP loading were shown to successfully encapsulate viable MSCs and maintain their survival over 28 days in vitro. GMP incorporation was also shown to modulate alkaline phosphatase production, but enhanced hydrogel mineralization along with higher polymer content even in the absence of cells. Moreover, the regenerative capacity of 2 mm thick hydrogels with GMPs only, MSCs only, or GMPs and MSCs was evaluated in vivo in an 8 mm rat critical size cranial defect for 4 and 12 weeks. GMP incorporation led to enhanced bony bridging and mineralization within the defect at each timepoint, and direct bone-implant contact as determined by microcomputed tomography and histological scoring, respectively. Encapsulation of both GMPs and MSCs enabled hydrogel degradation leading to ...Continue Reading

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Citations

Jun 23, 2016·Journal of Biomaterials Science. Polymer Edition·Tiffany N VoAntonios G Mikos
Aug 26, 2016·Journal of Biomedical Materials Research. Part a·Tiffany N VoAntonios G Mikos
Jul 8, 2016·The Journal of Bone and Joint Surgery. American Volume·Alexander M Tatara, Antonios G Mikos
Nov 3, 2016·Expert Opinion on Biological Therapy·Zhanpeng Zhang
Nov 24, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pariksha J KondiahViness Pillay
Feb 24, 2017·Advanced Materials·Shasha YaoRuikang Tang
Apr 28, 2017·Biomaterials Science·Zhichao HaoJun Wu
May 5, 2017·ACS Applied Materials & Interfaces·Zhaozhao DingDavid L Kaplan
Aug 31, 2017·Tissue Engineering. Part a·Andreas ArkudasRaymund E Horch
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Sep 10, 2019·Advanced Materials·Amanda L FacklamDaniel G Anderson
Nov 23, 2019·Journal of Biomedical Materials Research. Part a·Jason L GuoAntonios G Mikos
Sep 27, 2020·International Journal of Molecular Sciences·Tullio GenovaFederico Mussano
Mar 28, 2019·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Xiaoxiao WenLei Yang
Apr 1, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Chukwuma E Nweke, Jan P Stegemann
Jul 14, 2020·Frontiers in Bioengineering and Biotechnology·Zhitong ZhaoKee Woei Ng
Mar 7, 2020·Frontiers in Bioengineering and Biotechnology·Juliana R DiasAna L Oliveira
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Jul 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Arvind K Singh ChandelSuresh K Jewrajka
Oct 13, 2020·Frontiers in Bioengineering and Biotechnology·Guoke TangXiaojian Ye
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·K LavanyaN Selvamurugan
Jan 21, 2021·Tissue Engineering. Part a·Sean M BittnerAntonios G Mikos
Sep 6, 2020·Chinese Medical Journal·Wei ZhuXi-Sheng Weng
Jan 31, 2021·International Journal of Biological Macromolecules·Himansh GoelAditi Bhattacharya
Apr 7, 2021·Tissue Engineering. Part B, Reviews·Rehab N ShammaMagali Cucchiarini
May 1, 2021·Acta Biomaterialia·Jason L GuoAntonios G Mikos
Mar 30, 2021·Tissue Engineering. Part a·Payam ZarrintajMasoud Mozafari
Mar 14, 2019·ACS Applied Materials & Interfaces·Mehdi MehraliAlireza Dolatshahi-Pirouz

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