Injectable gel graft for bone defect repair.

Regenerative Medicine
Josephine FangBo Han

Abstract

To examine the performance of an injectable gel graft made of transglutaminase (Tg)-crosslinked gelatin gel with BMP-2 (BMP-2-Tg-Gel) for bone defect repair in animal models. BMP-2 mixed with gelatin gel was crosslinked using Tg. The release of tethered BMP-2 through autocrine and paracrine pathways was demonstrated by using C2C12 and NIH 3T3 cells, respectively. BMP-2-Tg-Gel was injected into the induced cranial defect site. After 14 days, the sample was removed for x-ray imaging and histological evaluation. Our in vivo results demonstrated that the injectable Tg-Gel with its osteoconductivity and controllable BMP-2 activity induced bone formation in our rat models when tethered with BMP-2. Tg-Gel as an injectable functional bone graft may enable the use of minimally invasive surgical procedures to treat irregular-shaped bone defects. Furthermore, this novel approach is capable of incorporating and controlling the release of therapeutic agents that may advance the science of tissue regeneration.

References

May 29, 2000·The Journal of Laryngology and Otology. Supplement·J M GrahamL Michaels
Jun 8, 2002·Advanced Drug Delivery Reviews·Haruya Sato
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bo HanMarcel E Nimni
Nov 11, 2003·Biomacromolecules·Tianhong ChenGregory F Payne
Mar 12, 2004·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hugo SchmoekelJeffrey A Hubbell
May 25, 2005·Biotechnology and Applied Biochemistry·Yoon Jeong ParkChong Pyoung Chung
Jun 2, 2005·Biomaterials·David Y S ChauMartin Griffin
Oct 20, 2005·Journal of Bioscience and Bioengineering·Akira ItoTakeshi Kobayashi
Nov 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Simon YoungAntonios G Mikos
Dec 26, 2006·Journal of Dental Research·Y LiuE B Hunziker
Jun 23, 2007·Journal of Biomedical Materials Research. Part a·C W YungT A Barbari
Jul 20, 2007·International Orthopaedics·William F McKayJeffrey M Badura
Oct 6, 2007·Advanced Drug Delivery Reviews·Angelo FontanaFrancesco M Veronese
Sep 18, 2009·Tissue Engineering. Part C, Methods·Kenrick KuwaharaBo Han
Dec 3, 2010·Neurosurgical Focus·Caroline SzpalskiStephen M Warren
May 3, 2011·Plastic and Reconstructive Surgery·Christopher R KinsellaJoseph E Losee
Feb 9, 2012·Advanced Drug Delivery Reviews·Maria-Pau GinebraEdgar B Montufar
Mar 30, 2012·Biomedical Materials·Jerald E DumasScott A Guelcher
Mar 30, 2012·Biomedical Materials·L AmbrosioR Giardino
May 1, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Soon Eon BaeDong Keun Han

❮ Previous
Next ❯

Methods Mentioned

BETA
zymography

Software Mentioned

InterGro
ImageJ

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.