Injectable platelet rich fibrin: cell content, morphological, and protein characterization

Clinical Oral Investigations
Hugo Almeida VarelaAurigena A Araújo

Abstract

The aim of the present study was to evaluate the blood cell content, morphological aspects, gene expression of type I collagen, and release of growth factors on an injectable platelet rich fibrin (i-PRF). Blood samples were collected from 15 volunteers to prepare i-PRF samples. Peripheral blood was used as a control group. Blood clot and i-PRF samples were cultured for 10 days. The supernatant of the samples was collected for ELISA immunoassay quantification of PDGF and VEGF growth factors over periods of 1, 8, 24, 72, and 240 h. I-PRF and blood clot samples were biologically characterized using histological and immunohistochemistry analysis for IL-10, osteocalcin, and TGF-β. Scanning electron microscopy (SEM) was used to inspect the fibrin network and distribution of blood platelets and leukocytes. Reverse transcriptase polymerase chain reaction (RT-PCR) method was used to evaluate gene expression for type I collagen. A higher concentration of platelets and lymphocytes was recorded in i-PRF than in peripheral blood (p < 0.05). The release of VEGF was higher in blood clot samples (1933 ± 704) than that for i-PRF (852 ± 376; p < 0.001). Immunohistochemistry showed upregulation of TGF-B, IL-10, and osteocalcin in the i-PRF group....Continue Reading

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Citations

Aug 27, 2019·Journal of Biomedical Materials Research. Part a·Jinglun ZhangYufeng Zhang
May 20, 2020·Journal of Functional Biomaterials·Chayane Karla Lucena de CarvalhoMauren Abreu de Souza
Feb 13, 2019·International Journal of Molecular Sciences·Dorottya KardosIstván Hornyák
Apr 16, 2020·Journal of Clinical Medicine·Giorgio SerafiniAlberto De Biase
Oct 18, 2020·Odontology·Hacer Sahin AydinyurtLevent Akinci
Nov 9, 2020·BMC Oral Health·Richard J MironYufeng Zhang
Nov 12, 2020·Advanced Materials·Izzet AltunRahmi Oklu
Aug 6, 2021·Journal of Microscopy and Ultrastructure·Sharmila JasmineMohammad A Alshuniaber
Sep 22, 2020·Journal of Oral Biology and Craniofacial Research·Amira M Elsherbini, Samah K Ezzat
Jun 7, 2020·Current Pharmaceutical Biotechnology·Azade RafieeMohammad H Morowvat

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