Injections of 6-hydroxydopamine in the substantia nigra of the rat brain: morphological and biochemical effects

Behavioural Brain Research
M BuonamiciP Bagnoli

Abstract

Rats with unilateral injections of 6-hydroxydopamine (6-OHDA) into the substantia nigra pars compacta were classified as active and inactive according to the intensity of their spontaneous and/or apomorphine-induced turning behavior (TB), and sacrificed at different survival times for morphological and biochemical analysis. In active rats, at any survival time, dopaminergic fluorescence in the nigrostriatal system as well as dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) content of the nucleus caudatus-putamen drastically decreased on the brain side ipsilateral to the injection. Dopaminergic fluorescence as well as DA and DOPAC content of the mesolimbic system ipsilateral to the injection also decreased. In inactive rats, at any survival time, 6-OHDA-induced lesions only partially involved both nigrostriatal and mesolimbic systems. Our results are indicative of a good correlation between the intensity of TB and the extent of 6-OHDA-induced lesions, as assessed by morphological and biochemical analysis.

References

Jan 15, 1978·European Journal of Pharmacology·C J Pycock, C D Marsden
Jan 1, 1976·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·U Ungerstedt
Jan 30, 1989·Neuroscience Letters·C A Altar, M R Marien
Jan 1, 1971·Acta Physiologica Scandinavica. Supplementum·U Ungerstedt
Nov 1, 1973·Experimental Neurology·D H York
Dec 1, 1968·European Journal of Pharmacology·U Ungerstedt
Jul 2, 1984·Life Sciences·M J Zigmond, E M Stricker
Jan 1, 1980·Neuroscience·C J Pycock
Oct 1, 1980·Journal of Neuroscience Methods·J C De la Torre
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E MelamedR J Wurtman
Oct 1, 1980·Journal of Comparative and Physiological Psychology·J F MarshallS Sawyer
Jan 1, 1981·Neuroscience·D van der KooyT Hattori
May 20, 1982·The Journal of Comparative Neurology·C R GerfenH C Fibiger
Jan 1, 1964·Acta Physiologica Scandinavica. Supplementum·C J CLEMEDSON, A JOENSSON

❮ Previous
Next ❯

Citations

Aug 8, 2002·Neurotoxicology·R H GwiazdaD R Smith
Dec 28, 2000·Behavioural Brain Research·R E JohnsonJ B Becker
Jun 1, 1994·Hippocampus·A Treves, E T Rolls

❮ Previous
Next ❯

Related Concepts

Related Feeds

Basal Ganglia

Basal Ganglia are a group of subcortical nuclei in the brain associated with control of voluntary motor movements, procedural and habit learning, emotion, and cognition. Here is the latest research.