Injury-induced synthesis and release of apolipoprotein E and clusterin from rat neural cells

The European Journal of Neuroscience
S Messmer-JoudrierA C Kato

Abstract

Apolipoproteins in the brain have assumed major clinical importance since it was shown that one of the allelic forms of apolipoprotein E, apoE-4, is a risk factor for Alzheimer's disease. Using tissue culture of embryonic rat spinal cord, we examined the effect of neuronal injury on the up-regulation of two apolipoproteins, apolipoprotein E and clusterin (apoJ). In order to study the influence of neuronal cells, we exploited the specific neurotoxic effect of elevated glutamate on these cells. Overstimulation by excess glutamate induced neuronal degeneration as assessed by morphological and biochemical criteria, notably the activity of choline acetyltransferase, which serves as a marker for cholinergic neurons. High concentrations of glutamate increased mRNA synthesis and the production and secretion of both apolipoprotein E and clusterin protein. Both neuronal cell death and release of the peptides were calcium-dependent and could be blocked by the NMDA receptor antagonist MK-801. Immunohistochemical data revealed the presence of clusterin in both neuronal and non-neuronal cells whereas apolipoprotein E was mainly expressed in non-neuronal cells. The results are suggestive of concerted up-regulation of apolipoprotein E and clus...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P S RoheimG L Vega
Nov 1, 1992·Journal of Neurobiology·D W Choi
Jan 1, 1992·Acta Neuropathologica·N H Choi-MiuraM Tomita
Apr 1, 1992·Trends in Biochemical Sciences·D E Jenne, J Tschopp
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J Y KohC W Cotman
Jan 1, 1991·Neuroscience·R F Regan, D W Choi
May 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·R W JamesD Hochstrasser
Jan 16, 1990·Biochimica Et Biophysica Acta·M C BlatterD Pometta
Oct 1, 1994·Neurochemistry International·K WellsL A Horrocks
Mar 3, 1994·The New England Journal of Medicine·S A Lipton, P A Rosenberg
Sep 18, 1993·Lancet·J PoirierS Gauthier
Feb 1, 1994·The Journal of Clinical Investigation·L E FrenchJ A Schifferli
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D E SchmechelA D Roses
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·W J StrittmatterA D Roses
Apr 30, 1993·Biochemical and Biophysical Research Communications·T WisniewskiB Frangione
Apr 1, 1993·Journal of Neurobiology·M K O'BryanM J Pearse

❮ Previous
Next ❯

Citations

Jun 18, 1999·Brain Research Bulletin·K R PennypackerP R Sanberg
Sep 23, 2003·Critical Care Medicine·Mary E KerrDonald W Marion
Sep 18, 2008·Neuroreport·Grzegorz WicherHakan Aldskogius
May 5, 2005·The European Journal of Neuroscience·Grzegorz K Wicher, Håkan Aldskogius
Oct 30, 2003·Multiple Sclerosis : Clinical and Laboratory Research·D KarussisJ Chapman
Feb 16, 2011·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·M D CusimanoM Freedman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: APOE

Apolipoprotein E (APOE) polymorphic alleles are major genetic risk factors for Alzheimer's disease. Discover the latest research on APOE and other genetic determinants of Alzheimer's disease here.

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.