Inkjet-Printing Patterned Chip on Sticky Superhydrophobic Surface for High-Efficiency Single-Cell Array Trapping and Real-Time Observation of Cellular Apoptosis

ACS Applied Materials & Interfaces
Yingnan SunShusheng Zhang

Abstract

Single-cell assays have broad applications in cellular studies, tissue engineering, fundamental studies of cell-cell interactions, and understanding of cell-to-cell variations. Most existing methods for micron-sized cell patterning are still based on lithography-based microfabrication process. Thus, exploiting new mask-free strategies while maintaining high-precision single-cell patterning is still a great challenge. Here, we presented a facile, low-cost, and mask-free approach for constructing high-resolution patterning on sticky superhydrophobic (SH) substrates based on inkjet printing with ordinary precision. In this work, the SH surface with both high contact angle and relatively high contact angle hysteresis can not only obtain high-resolution spots but also avoid droplets bouncing behavior. We improved the feature size of printed protein spots as small as 4 μm, which is much smaller than protein spots used for single-cell trapping. Moreover, with the assistance of a narrow microchannel, the inkjet-printing patterned chip with fibronectin ink allows for fast and high-efficiency trapping of multiple single-cell arrays. Using this method, single-cell occupancy could reach approximately 81% within 30 min on subcellular-sized ...Continue Reading

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Citations

Aug 14, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuemeng YangShutao Wang
Jan 20, 2021·ACS Biomaterials Science & Engineering·Vittorio FerraraBruno Pignataro
Mar 7, 2021·International Journal of Pharmaceutics·Susannah Elizabeth EvansRonan Daly
Jul 23, 2021·Progress in Biophysics and Molecular Biology·Xiaolu ZhuFang Teng

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis