Innate IFN-γ-producing cells in the spleen of mice early after Listeria monocytogenes infection: importance of microenvironment of the cells involved in the production of innate IFN-γ.

Frontiers in Immunology
Koichi Kubota, Yuichi Kadoya

Abstract

Production of innate interferon-γ (IFN-γ) is a crucial step in immunological defense against bacteria. However, there is little information regarding cellular mechanisms underlying IFN-γ production in vivo early after bacterial infection. Here we analyze innate IFN-γ production in the spleen of mice early after Listeria monocytogenes (LM) infection ex vivo by flow-cytometry and in situ by immunohistochemistry, and compare them with the IFN-γ-producing cells reported previously in our in vitro coculture system in which cell-cell interaction between lymphocytes and dying bacterial-infected macrophages is required for the production of IFN-γ. In the spleen at 20 h after LM infection, natural killer (NK) cells, a subset of αβ T cells, and subsets of NKT and γδ T cells produced IFN-γ with features similar to the IFN-γ-producing cells in our in vitro coculture system. Immunohistochemistry revealed that LM bacteria were first phagocytosed mainly by ER-TR9⁺ marginal zone macrophages (MZMs), then forming infectious foci in close vicinity of the marginal zone (MZ) at 20-h postinfection. At this time point, the IFN-γ-producing cells were accumulating at the same site of infectious foci, around which ER-TR9⁺ MZMs were clustered but most of...Continue Reading

Citations

Dec 4, 2013·Cell Research·Yan BaoXuetao Cao
Jun 9, 2016·PloS One·Jamal QualaiMeritxell Genescà
Oct 26, 2021·Frontiers in Immunology·Rui DaiYiping Yang

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Methods Mentioned

BETA
FACS
flow-cytometry
dot blots

Software Mentioned

Hazebuster
IPlab
CellQuest

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