Innate Immune Responses in the Neutrophils of Community Dwelling and Nursing Home Elders

Journal of Aging Science
Manisha Juthani-MehtaRuth R Montgomery

Abstract

To evaluate innate immune responses of older disabled nursing home residents that may contribute to infectious disease susceptibility, we compared surface markers and signaling efficiency of neutrophils from nursing home residents and community dwelling elders. Observational pilot study. Five New Haven, CT area nursing homes and the greater New Haven community. 15 nursing home residents and 43 community dwelling elders. Neutrophils were isolated and Toll-like receptor (TLR) and β2 integrin expression on the surface of unstimulated neutrophils were measured via flow cytometry. Chemokine induction was determined by Quantitative PCR. Surface expression of TLR4 was elevated among nursing home residents compared to community dwellers (mean percent positive cells 33.91 [SE 2.75] vs. 15.67 [SE 1.58], p<0.001), while expression of the β2 integrins CD11b and CD18 was significantly lower (mean fluorescent intensity 460.8 [SE 49.1] vs. 632.9 [SE 29.5] for CD11b and 59.6 [SE 7.9] vs. 137.6 [SE 4.6] for CD18, p<0.0001). Neutrophils from nursing home residents produced substantially reduced levels of chemokines at baseline and after stimulation. Because integrins are an important pathway to phagocyte signaling and contribute to adherence and...Continue Reading

Citations

Jun 29, 2014·Immunology Letters·Kamran Haq, Janet E McElhaney
Apr 26, 2016·Cell Biology International·Joes Nogueira-NetoKarin A Simon
Sep 3, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Daisy WilsonElizabeth Sapey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.