Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs

Veterinary Research
Viktor AhlbergCaroline Fossum

Abstract

Saponin-based adjuvants have been widely used to enhance humoral and cellular immune responses in many species, but their mode of action is not fully understood. A characterization of the porcine transcriptional response to Matrix-M was performed in vitro using lymphocytes, monocytes or monocyte-derived dendritic cells (MoDCs) and in vivo. The effect of Matrix-M was also evaluated in specific pathogen free (SPF) pigs exposed to conventionally reared pigs. The pro-inflammatory cytokine genes IL1B and CXCL8 were up-regulated in monocytes and lymphocytes after Matrix-M exposure. Matrix-M also induced IL12B, IL17A and IFNG in lymphocytes and IFN-α gene expression in MoDCs. Several genes were indicated as up-regulated by Matrix-M in blood 18 h after injection, of which the genes for IFN-α and TLR2 could be statistically confirmed. Respiratory disease developed in all SPF pigs mixed with conventional pigs within 1-3 days. Two out of four SPF pigs injected with saline prior to contact exposure displayed systemic symptoms that was not recorded for the four pigs administered Matrix-M. Granulocyte counts, serum amyloid A levels and transcription of IL18 and TLR2 coincided with disease progression in the pigs. These results support furthe...Continue Reading

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Citations

Jan 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juliane Deise FleckSimone Gasparin Verza
Mar 6, 2019·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Flavourings (FAF)Peter Moldeus
Sep 26, 2020·Frontiers in Immunology·Sana O AlturkiElias K Haddad
May 18, 2020·Current Diabetes Reviews·Neeraj ChoudharyAshish Suttee

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Methods Mentioned

BETA
FCS
electrophoresis
PCR
Assay
ELISA

Software Mentioned

geNorm
ISCOM
GraphPad
Matrix
Prism
qBasePLUS
Excel

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