Innate inflammatory gene expression profiling in potential brain-dead donors: detailed investigation of the effect of common corticosteroid therapy

Innate Immunity
Reza GholamnezhadjafariAbbas Rezaei

Abstract

Our study aimed to assess the influence of common methylprednisolone therapy on innate inflammatory factors in potential brain-dead organ donors (BDDs). The study groups consisted of 50 potential BDDs who received 15 mg/kg/d methylprednisolone and 25 live organ donors (LDs) as control group. Innate immunity gene expression profiling was performed by RT-PCR array. Soluble serum cytokines and chemokines, complement components, heat shock protein 70 (HSP70) and high mobility group box-1 (HMGB1) were measured by ELISA. Surface expression of TLR2 and TLR4 were determined using flow cytometry. Gene expression profiling revealed up-regulation of TLRs 1, 2, 4, 5, 6, 7 and 8, MYD88, NF-κB, NF-κB1A, IRAK1, STAT3, JAK2, TNF-α, IL-1β, CD86 and CD14 in the BDD group. Remarkably, the serum levels of C-reactive protein and HSP70 were considerably higher in the BDD group. In addition, serum amounts of IL-1β, IL-6, TNF-α, HMGB1, HSP70, C3a and C5a, but not IL-8, sCD86 or monocyte chemoattractant protein-1, were significantly increased in the BDD group. Significant differences were observed in flow cytometry analysis of TLR2 and TLR4 between the two groups. In summary, common methylprednisolone therapy in BDDs did not adequately reduce systemic ...Continue Reading

References

Jan 1, 1996·Annual Review of Immunology·A S Baldwin
May 1, 1997·British Journal of Anaesthesia·E G McKeatingL Mascia
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Jul 21, 2000·Annals of Surgery·J PratschkeN L Tilney
Jun 1, 2002·Science·David S Aaronson, Curt M Horvath
Oct 14, 2004·Transplantation·Willemijn N NijboerRutger J Ploeg
Mar 12, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Vassilios S AvlonitisJohn H Dark
Apr 6, 2005·Transplantation Proceedings·W N NijboerR J Ploeg
Apr 6, 2005·Transplantation Proceedings·O KuecuekJ Pratschke
Nov 4, 2005·American Journal of Physiology. Cell Physiology·Jong Sung ParkEdward Abraham
Dec 14, 2006·Transplantation·Dmitri NovitzkyH Myron Kauffman
Jan 16, 2007·Vascular Pharmacology·Belay Tesfamariam, Albert F DeFelice
Jun 13, 2009·Acta Anaesthesiologica Scandinavica·M CanS Acikgoz
Aug 12, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Qing-Wu YangJing-Zhou Wang
Dec 18, 2010·Journal of the American Society of Nephrology : JASN·Markus WahrmannGeorg A Böhmig
Jul 6, 2012·Journal of Critical Care·Rajat DharRobert Wright
Dec 12, 2012·Nature Reviews. Gastroenterology & Hepatology·Yuan ZhaiJerzy W Kupiec-Weglinski
Jan 30, 2013·Journal of Translational Medicine·Leon F A van DullemenHenri G D Leuvenink
Oct 17, 2013·Clinical Transplantation·Dorottya K de VriesAlexander F M Schaapherder
Jun 25, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J SmedbråtenS Thiel

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Citations

Jan 31, 2018·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Shiwei MaFeng Lv
May 14, 2020·Molecular Genetics & Genomic Medicine·Reza JafariReza Aflatoonian

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Methods Mentioned

BETA
biopsies
density
flow cytometry
Profiler
PCR
ELISA
hormone-replacement therapy

Software Mentioned

FlowJo
GraphPad Prism
multisKan spectrum
CELLQuest
Prism

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