Innervation-dependent and fiber type-specific transcriptional regulation of the slow myosin heavy chain 2 promoter in avian skeletal muscle fibers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Hongbin JiangJ X DiMario

Abstract

Skeletal muscle fiber type is regulated, in part, by innervation leading to transcriptional regulation of fiber type-specific genes. Here, we report the initial characterization of the transcriptional regulation of the slow myosin heavy chain 2 (MyHC2) promoter in innervated and noninnervated slow medial adductor (MA) and fast pectoralis major (PM) muscle fibers in cell culture. The proximal 1358 bp of slow MyHC2 upstream DNA contains a functional E-box and binding sites for myocyte enhancer factor 2 (MEF2) and nuclear factor of activated T cells (NFAT). Mutagenesis studies indicated that both MEF2 and NFAT binding sites are required for innervation-induced slow MyHC2 promoter activity in MA muscle fibers. However, MEF2 transcription factor activity was unaffected by innervation and did not demonstrate fiber type-specific interactions with the slow MyHC2 MEF2 binding site. NFAT transcription factor activity did increase in innervated MA muscle fibers and not in PM muscle fibers, indicating innervation and muscle fiber type-specific regulation. However, transfection of constitutively active NFAT indicated that NFAT is insufficient to induce slow MyHC2 gene expression in either fast PM or slow MA muscle fibers without innervation...Continue Reading

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Citations

Feb 19, 2008·Cell and Tissue Research·Yoshikazu Matsuoka, Akio Inoue
Dec 29, 2015·Biochemical and Biophysical Research Communications·Kristina Weimer, Joseph X DiMario
Jan 15, 2010·Experimental Cell Research·Jennifer R CrewJoseph X DiMario
May 25, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kristina WeimerJoseph X DiMario
Oct 13, 2006·Cell Calcium·M Angélica Carrasco, Cecilia Hidalgo
Jul 19, 2016·Biochimica Et Biophysica Acta·Eric J Cavanaugh, Joseph X DiMario
Nov 18, 2006·Journal of Cellular Physiology·Joachim D MeissnerRenate J Scheibe
Jun 23, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nobuko HagiwaraAnn Liu
Jan 11, 2008·American Journal of Physiology. Cell Physiology·Juan Antonio ValdésM Angélica Carrasco
Feb 4, 2011·Journal of Muscle Research and Cell Motility·Jillian Theobald, Joseph X DiMario

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