Innovative biodegradable poly(L-lactide)/collagen/hydroxyapatite composite fibrous scaffolds promote osteoblastic proliferation and differentiation

International Journal of Nanomedicine
Guoqiang ZhouJinchao Zhang

Abstract

The development of an artificial bone graft which can promote the regeneration of fractures or diseased bones is currently the most challenging aspect in bone tissue engineering. To achieve the purpose of promoting bone proliferation and differentiation, the artificial graft needs have a similar structure and composition of extracellular matrix. One-step electrospinning method of biocomposite nanofibers containing hydroxyapatite (HA) nanoparticles and collagen (Coll) were developed for potential application in bone tissue engineering. Nanocomposite scaffolds of poly(L-lactide) (PLLA), PLLA/HA, PLLA/Coll, and PLLA/Coll/HA were fabricated by electrospinning. The morphology, diameter, elements, hydrophilicity, and biodegradability of the composite scaffolds have been investigated. The biocompatibility of different nanocomposite scaffolds was assessed using mouse osteoblasts MC3T3-E1 in vitro, and the proliferation, differentiation, and mineralization of cells on different nanofibrous scaffolds were investigated. The results showed that PLLA/Coll/HA nanofiber scaffolds enhanced cell adhesion, spreading, proliferation, differentiation, mineralization, and gene expression of osteogenic markers compared to other scaffolds. In addition...Continue Reading

Citations

Aug 1, 2019·Biomaterials Science·Anastasiia KashirinaJinsong Leng
May 12, 2018·Genes·Julia ScheinpflugFrank Schulze
Jul 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rohaya Megat Abdul WahabFarinawati Yazid
Dec 23, 2020·International Journal of Biological Macromolecules·Bhasha SharmaPurnima Jain
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Francilio Carvalho OliveiraAnderson Oliveira Lobo

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BETA
X-ray
dynamic light scattering

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Nano Measurer

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