Innovative Comprehensive Experiments to Study the Morphological Changes of Apoptotic Cells for Undergraduate Education

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
Qing SongYun Bai

Abstract

This innovative comprehensive experiment is a compulsory course for third-year undergraduate students in the eight-year training program leading to a Doctor of Medicine degree or Doctor of Philosophy degree at Peking University Health Science Center (PKUHSC). There is a critical educational need to prepare these students for the increasing accessibility of research experience. This experimental curriculum of medical cell biology was developed to fulfill such a requirement, which consists of three experiments, including the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium) assay and the immunofluorescence and comet assays to observe antitumor drug-induced cell apoptosis. This experiment-based teaching system provides the participating students an opportunity to enhance their understanding of important biological research techniques and the instrumentation involved and to foster a better understanding of the research process within the classroom. Upon completion of the course, students' feedback showed that the curriculum increased their confidence in performing molecular techniques, and they reported positively on doing a research project in class. This course helped students improve their understand...Continue Reading

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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