Abstract
This innovative comprehensive experiment is a compulsory course for third-year undergraduate students in the eight-year training program leading to a Doctor of Medicine degree or Doctor of Philosophy degree at Peking University Health Science Center (PKUHSC). There is a critical educational need to prepare these students for the increasing accessibility of research experience. This experimental curriculum of medical cell biology was developed to fulfill such a requirement, which consists of three experiments, including the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium) assay and the immunofluorescence and comet assays to observe antitumor drug-induced cell apoptosis. This experiment-based teaching system provides the participating students an opportunity to enhance their understanding of important biological research techniques and the instrumentation involved and to foster a better understanding of the research process within the classroom. Upon completion of the course, students' feedback showed that the curriculum increased their confidence in performing molecular techniques, and they reported positively on doing a research project in class. This course helped students improve their understand...Continue Reading
References
Sep 1, 1971·The Journal of Pathology·J F Kerr
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·E RojasM Valverde
Mar 13, 1999·Biochemical Pharmacology·D A Gewirtz
Mar 20, 2001·Nature Reviews. Molecular Cell Biology·M P Mattson
Aug 6, 2003·Life Sciences·W P TsangT T Kwok
Mar 9, 2004·Molecular Biotechnology·Andrew R Collins
Jun 1, 2004·Pharmacological Reviews·Giorgio MinottiLuca Gianni
Oct 15, 2010·Anais Brasileiros De Dermatologia·Valéria AokiZilda N P Oliveira
Sep 29, 2011·Journal of Experimental & Clinical Cancer Research : CR·Rebecca S Y Wong
Jul 31, 2013·Methods in Molecular Biology·Diana AndersonJulian Laubenthal
Sep 10, 2014·Frontiers in Genetics·Amaya AzquetaAndrew Collins
Feb 11, 2015·Oncology Letters·Qiao-Mei DongLi Zhao
Feb 22, 2017·Indian Dermatology Online Journal·Varsha M ShettyRaghavendra Rao
Oct 3, 2017·Cell Calcium·Geert Bultynck, Jan B Parys
Feb 13, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qingfang HanAihua Li
Apr 3, 2018·Acta histochemica·Ewa GrelaAgnieszka Grabowiecka
Jul 20, 2018·Molecular Biology Reports·Pavlína Majtnerová, Tomáš Roušar