Innovative Cryopreservation Process Using a Modified Core/Shell Cell-Printing with a Microfluidic System for Cell-Laden Scaffolds

ACS Applied Materials & Interfaces
Jae Yoon LeeGeunHyung Kim

Abstract

This work investigated the printability and applicability of a core/shell cell-printed scaffold for medium-term (for up to 20 days) cryopreservation and subsequent cultivation with acceptable cellular activities including cell viability. We developed an innovative cell-printing process supplemented with a microfluidic channel, a core/shell nozzle, and a low-temperature working stage to obtain a cell-laden 3D porous collagen scaffold for cryopreservation. The 3D porous biomedical scaffold consisted of core/shell struts with a cell-laden collagen-based bioink/dimethyl sulfoxide mixture in the core region and an alginate/poly(ethylene oxide) mixture in the shell region. Following 2 weeks of cryopreservation, the cells (osteoblast-like cells or human adipose stem cells) in the scaffold showed good viability (over 90%), steady growth, and mineralization similar to those of a control scaffold fabricated using a conventional cell-printing process without cryopreservation. We believe that these results are attributable to the optimized fabrication processes the cells underwent, including safe freezing/thawing processes. On the basis of these results, this fabrication process has great potential for obtaining core/shell cell-laden colla...Continue Reading

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Citations

Mar 14, 2019·Advanced Healthcare Materials·Lei ShaoYong He
May 28, 2019·Journal of Biomaterials Science. Polymer Edition·XuanQi ZhengAiMin Wu
Nov 28, 2018·Micromachines·Junyao WangTian-Yi Su
Apr 16, 2020·Advanced Biosystems·Roaa AlnemariMohammad A Qasaimeh

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