Innovative mixture of salts in the quick, easy, cheap, effective, rugged, and safe method for the extraction of residual macrolides in milk followed by analysis with liquid chromatography and tandem mass spectrometry

Journal of Separation Science
Rafaela Pinto da CostaArmi Wanderley da Nóbrega

Abstract

A simple extraction technique has been developed for seven macrolide antibiotics in milk. The procedure involves a modified quick, easy, cheap, effective, rugged, and safe method based on acetonitrile extraction, followed by the addition of a mixture of salts (sodium sulfate, sodium chloride, and potassium carbonate) not yet reported in literature. The method was validated for tylosin and was selective, free of matrix effect, and linear in the range of 0.78-18.75 ng/mL in the final extract, corresponding to 0.125-3 times the maximum residue limit. The limit of detection, limit of quantification, decision limit, and detection capability were, respectively, 0.84, 2.79, 58.4, and 71.7 μg/kg. The overall average recovery at 25, 50, and 75 μg/kg ranged from 89-97%. Repeatability and intermediate precision expressed by relative standard deviations were below 10.5 and 12%, respectively. The extension of the validation for spiramycin, throleandomycin, oleandomycin, roxithromycin, erythromycin, and clarithromycin is under consideration since the procedure proved to be able to efficiently extract all studied macrolides, with recoveries from 74-104% at 50 μg/kg for tylosin, erythromycin, spiramycin, and oleandomycin and 20 μg/kg for throl...Continue Reading

References

Mar 20, 2008·Annual Review of Public Health·Ellen K SilbergeldLance B Price
Apr 17, 2008·Rapid Communications in Mass Spectrometry : RCM·Sherri B TurnipseedKeith E Miller
Aug 30, 2008·Journal of Chromatography. a·M M Aguilera-LuizAntonia Garrido Frenich
Dec 30, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yu-Yun TangDeng-Fwu Hwang
Jun 13, 2012·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Takayuki NakajimaIchiro Takano
Apr 2, 2014·The Veterinary Journal·Satu PyöräläKarolina Törneke

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