Inntags: small self-structured epitopes for innocuous protein tagging

Nature Methods
Maya V GeorgievaMartí Aldea

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

References

May 25, 1977·Journal of Molecular Biology·F C BernsteinM Tasumi
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·X LiuH F Lodish
Dec 21, 2000·Trends in Cell Biology·R R Kopito
Oct 27, 2004·Journal of Structural and Functional Genomics·Esmeralda A WoestenenkHelena Berglund
Jun 1, 2005·Trends in Biotechnology·David S Waugh
Feb 4, 2006·Molecular Cell·Richelle SopkoBrenda Andrews
Nov 14, 2006·Nucleic Acids Research·Gil AlterovitzMarco F Ramoni
Feb 1, 2007·Nature Methods·Laura SchembriFrancesca De Giorgi
Mar 21, 2007·Nature Methods·Simon FredrikssonRonald W Davis
Sep 5, 2007·Current Opinion in Biotechnology·Mike Cook, Mike Tyers
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Brian D SlaughterRong Li
Jan 8, 2008·Nature Methods·Xiaodong ZhangZhenghe Wang
Nov 13, 2009·Nucleic Acids Research·Randi VitaBjoern Peters
Jan 27, 2010·Biochimica Et Biophysica Acta·Bin XueVladimir N Uversky
Jun 1, 2010·Molecular & Cellular Proteomics : MCP·Maryam AfkarianTowia A Libermann
Dec 1, 2011·Nucleic Acids Research·Marco PuntaRobert D Finn
Apr 10, 2012·Nature Methods·Dirk LandgrafJohan Paulsson
Aug 23, 2012·Nature Communications·Francisco FerrezueloMartí Aldea
Sep 20, 2012·Trends in Biochemical Sciences·Peter Tompa
Nov 20, 2012·Nucleic Acids Research·J A BlakeM Westerfield
Jun 12, 2013·Annual Review of Biochemistry·Yujin E KimF Ulrich Hartl
Jan 1, 2014·Molecular Cell·Galal YahyaMartí Aldea
Oct 17, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Neus PedrazaCarme Gallego

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Citations

Jun 18, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E Christopher PartridgeEric M Mendenhall
Aug 16, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Diana B PeckysNiels de Jonge
Nov 29, 2016·Scientific Reports·Leonard BurgDarius Balciunas
Aug 15, 2019·Nucleic Acids Research·Goran JosipovićVlatka Zoldoš
Apr 17, 2019·Life Science Alliance·David F MorenoMartí Aldea
Sep 29, 2019·Nature Communications·Hansjörg GötzkeSteffen Frey
Aug 2, 2018·The Journal of Physical Chemistry Letters·Jing ZhangPing Wang
Dec 10, 2016·ACS Infectious Diseases·Miroslava StrmiskovaJohn Paul Pezacki

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