Inoculation and co-inoculation of alfalfa seedlings with root growth promoting microorganisms (Piriformospora indica , Glomus intraradices and Sinorhizobium meliloti ) affect molecular structures, nutrient profiles and availability of hay for ruminants

Animal Nutrition
Milad JafariArjan Jonker

Abstract

Inoculation of alfalfa seedlings with root growth promoting microorganisms under semi-arid climate condition may improve biomass production and nutritive value. The current study aimed to investigate the effect of inoculation of alfalfa seedlings with Piriformospora indica (Pi) and co-inoculating Pi with Glomus intraradices (Gi + Pi) or Sinorhizobium meliloti (Sm + Pi) on hay yield, chemical composition, molecular structures by Fourier transformed infrared (FTIR) spectroscopy, in situ ruminal degradability and in vitro gas production. Seedlings were grown in experimental pots in a greenhouse until first cut and then transferred outside and cut a further 4 times. Biomass yield was similar across the treatments. Acid detergent fiber (ADF) concentration was higher in Pi than in control hay, and ADF decreased further with co-inoculation (P < 0.05). The ether extract (EE) concentration was lower for Pi and Gi + Pi compared with control hay, and control, Pi and Gi + Pi hays had lower EE concentration compared with Sm + Pi (P < 0.05). The FTIR spectroscopic vibration peak height ratio related to proteins (amide 1 + amide 2): total carbohydrate ratio was lower for the inoculation treatments compared with control hay (P < 0.05). In situ...Continue Reading

Citations

Mar 11, 2020·International Journal of Molecular Sciences·Lei TianChunjie Tian

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Methods Mentioned

BETA
infrared spectroscopy
environmental stress

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SAS
ANKOM
Statistica

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