Inoculum selection influences the biochemical methane potential of agro-industrial substrates

Microbial Biotechnology
Jo De VriezeNico Boon

Abstract

Obtaining a reliable estimation of the methane potential of organic waste streams in anaerobic digestion, for which a biochemical methane potential (BMP) test is often used, is of high importance. Standardization of this BMP test is required to ensure inter-laboratory repeatability and accuracy of the BMP results. Therefore, guidelines were set out; yet, these do not provide sufficient information concerning origin of and the microbial community in the test inoculum. Here, the specific contribution of the methanogenic community on the BMP test results was evaluated. The biomethane potential of four different substrates (molasses, bio-refinery waste, liquid manure and high-rate activated sludge) was determined by means of four different inocula from full-scale anaerobic digestion plants. A significant effect of the selected inoculum on the BMP result was observed for two out of four substrates. This inoculum effect could be attributed to the abundance of methanogens and a potential inhibiting effect in the inoculum itself, demonstrating the importance of inoculum selection for BMP testing. We recommend the application of granular sludge as an inoculum, because of its higher methanogenic abundance and activity, and protection fro...Continue Reading

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Citations

Oct 30, 2015·Bioresource Technology·Kaoutar AboudiLuis Isidoro Romero-García
Jul 31, 2016·Bioresource Technology·Ricardo Luis Radis SteinmetzHugo Moreira Soares
Oct 12, 2018·Molecular Ecology·Pawel SierocinskiAngus Buckling
Jul 11, 2018·Biotechnology for Biofuels·Cristina Gonzalez-FernandezMercedes Ballesteros
Nov 16, 2019·The Science of the Total Environment·E Fernández-PalaciosD Gabriel

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Methods Mentioned

BETA
PCR

Software Mentioned

StepOnePlus
SPSS Statistics

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